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HPV Type 16 E6 Antibody (PA5-117355) in WB
Western blot analysis of HPV Type 16 E6 in Various whole cell extracts (30 µg). Samples were separated with 15% SDS-PAGE and incubated in HPV Type 16 E6 polyclonal antibody (Product # PA5-117355) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
Product Details
PA5-117355
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length Human Papilloma virus type 16 E6 recombinant protein
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901985
Product Specific Information
Centrifuge briefly prior to opening.
Target Information
Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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