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Application
FIGURE: 1 / 1
HRSP12 Antibody (PA5-96286) in WB
Western blot analysis of HRSP12 in extracts of various cell lines. Samples were incubated with HRSP12 Polyclonal antibody (Product # PA5-96286) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96286
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human HRSP12 (NP_0058271)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808088
Product Specific Information
Immunogen sequence: MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL; Positive Samples: MCF-7, K-562, Mouse liver, Mouse kidney, Rat kidney; Cellular Location: Cytoplasm, Nucleus
Target Information
Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14.5 kDa translational inhibitor protein; 2-iminobutanoate/2-iminopropanoate deaminase; Heat-responsive protein 12; hp14.5; L-PSP; Liver perchloric acid-soluble protein; perchloric acid soluble protein; perchloric acid-soluble protein; perchrolic acid soluble protein; Reactive intermediate imine deaminase A homolog; reactive intermediate/imine deaminase A homolog; ribonuclease UK114; rp14.5; Translation inhibitor L-PSP ribonuclease; translational inhibitor p14.5; translational inhibitor protein p14.5; UK114 antigen homolog
Gene Aliases: HR12; HRP12; HRSP12; P14.5; PSP; PSP1; RIDA; UK114
UniProt ID: (Mouse) P52760, (Rat) P52759, (Human) P52758
Entrez Gene ID: (Mouse) 15473, (Rat) 65151, (Human) 10247
nuclease activity
endonuclease activity
hydrolase activity
endoribonuclease activity, producing 3'-phosphomonoesters
deaminase activity
long-chain fatty acid binding
phosphoric ester hydrolase activity
protein homodimerization activity
ion binding
T/G mismatch-specific endonuclease activity
poly(A) RNA binding
retroviral 3' processing activity
transition metal ion binding
platinum binding
xenon atom binding
deaminase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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