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Application
FIGURE: 1 / 7
HSP40 Antibody (MA5-25375) in ICC/IF
Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Mouse Monoclonal Antibody (OTI1G4) (Product # MA5-25375) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at ... View More
Product Details
MA5-25375
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI1G4
Immunogen
Full length human recombinant protein of DNAJB1 produced in HEK293T cell
Conjugate
Unconjugated
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2724490
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B
Gene Aliases: DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1
UniProt ID: (Human) P25685
Entrez Gene ID: (Human) 3337, (Dog) 476688
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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