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            • Primary Antibodies ›
            • HSP60 Antibodies

            NeoBiotechnologies

            HSP60 (Heat Shock Protein 60) Monoclonal Antibody (HSPD1, 875)

            View all (94) HSP60 antibodies
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            Datasheet
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            Cite HSP60 (Heat Shock Protein 60) Monoclonal Antibody (HSPD1, 875)

            • Antibody Testing Data (4)
            Application
            HSP60 (Heat Shock Protein 60) Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 4

            HSP60 (Heat Shock Protein 60) Antibody (3329-MSM5-P1) in ICC/IF

            Immunocytochemistry/Immunofluorescence analysis of MeOH-fixed Hela cells using HSP60 (Heat Shock Protein 60) Monoclonal Antibody (Product # 3329-MSM5-P1) followed by goat anti-mouse IgG-CF488 (green).Nuclei stained with RedDot..
            View Product

            HSP60 (Heat Shock Protein 60) Antibody in Immunocytochemistry (ICC/IF)
            HSP60 (Heat Shock Protein 60) Antibody in Immunocytochemistry (ICC/IF)
            HSP60 (Heat Shock Protein 60) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            HSP60 (Heat Shock Protein 60) Antibody in Peptide array (ARRAY)

            Product Details

            3329-MSM5-P1

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1-2 µg/mL
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1-2 µg/mL
            -

            Immunocytochemistry (ICC/IF)

            1-2 µg/mL
            -

            Peptide Array (Array)

            Assay-dependent
            -
            Product Specifications

            Species Reactivity

            Chicken, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rat

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            HSPD1, 875

            Immunogen

            Recombinant full-length human HSPD1 protein
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            200 µg/mL

            Purification

            Protein A/G

            Storage buffer

            PBS, pH 7.4, with 0.05% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

            Target Information

            This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; hsp60; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1

            View more View less

            Gene Aliases: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; RCJMB04_7g5; SPG13

            View more View less

            UniProt ID: (Chicken) Q5ZL72, (Human) P10809, (Chinese hamster) P18687, (Mouse) P63038, (Rat) P63039

            View more View less

            Entrez Gene ID: (Chicken) 424059, (Human) 3329, (Chinese hamster) 100689473, (Mouse) 15510, (Rat) 63868

            View more View less

            Function(s)

            lipopolysaccharide binding p53 binding double-stranded RNA binding protein binding ATP binding high-density lipoprotein particle binding isomerase activity ubiquitin protein ligase binding apolipoprotein A-I binding chaperone binding DNA replication origin binding single-stranded DNA binding ATPase activity poly(A) RNA binding unfolded protein binding nucleotide binding protease binding protein complex binding insulin binding protein heterodimerization activity misfolded protein binding

            Process(es)

            B cell cytokine production MyD88-dependent toll-like receptor signaling pathway positive regulation of T cell mediated immune response to tumor cell protein folding activation of cysteine-type endopeptidase activity involved in apoptotic process apoptotic mitochondrial changes response to cold positive regulation of interferon-alpha production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production mitochondrial unfolded protein response protein refolding B cell proliferation T cell activation positive regulation of macrophage activation negative regulation of apoptotic process protein import into mitochondrial intermembrane space isotype switching to IgG isotypes protein stabilization positive regulation of T cell activation interaction with symbiont cellular response to interleukin-7 detection of misfolded protein response to unfolded protein B cell activation positive regulation of apoptotic process negative regulation of neuron apoptotic process positive regulation of inflammatory response chaperone mediated protein folding requiring cofactor 'de novo' protein folding viral process chaperone-mediated protein complex assembly protein maturation response to hypoxia response to heat response to organic substance response to organic cyclic compound response to lipopolysaccharide response to ATP response to cocaine response to drug response to hydrogen peroxide response to estrogen
            It has to be done as per old AB suggested Products section.
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