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Invitrogen
HSP70 Monoclonal Antibody (W27)
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HSP70 Antibody (MA1-90504)
Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A549 cells were transfected with HSP70 siRNA and decrease in signal intensity was observed in western blot application using HSP70 Antibody (Product # MA1-90504).
Product Details
MA1-90504
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Non-human primate
Published species
Dog,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
W27
Immunogen
HSP70 protein from HeLa cells.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1955811
Product Specific Information
Recommended positive controls: BT474 or HeLa cells. Breast carcinoma..
Target Information
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 70 kda heat shock protein-2; 70 kilodalton heat shock protein; chaperone; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kD protein 3; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 3; heat shock 70kDa protein 4; Heat shock protein; Heat Shock Protein 70; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70
Gene Aliases: APG-2; APG2; HEL-S-103; HEL-S-5a; HS24/P52; HSP70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; HSP70I; hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA3; HSPA4; HSPH2; HSX70; hum70t; RY
UniProt ID: (Human) P0DMV8, (Bovine) Q27975, (Human) O75634, (Bovine) P0CB32, (Human) P54652, (Bovine) P34933, (Human) Q2TAL4
Entrez Gene ID: (Human) 3303, (Bovine) 282254, (Human) 3304, (Human) 3305, (Bovine) 540190, (Human) 3306, (Bovine) 281827, (Bovine) 536558, (Human) 3308
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