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Invitrogen
HYOU1 Polyclonal Antibody
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Application
FIGURE: 1 / 11
HYOU1 Antibody (PA5-27655) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HYOU1 was performed in U2OS cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HYOU1 Polyclonal Antibody (Product # PA5-27655) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-27655
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.41 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545131
Product Specific Information
Recommended positive controls: MCF-7, MDA-MB-231, mouse liver, rat brain.
Predicted reactivity: Mouse (92%), Rat (90%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; FLJ94899; FLJ97572; Grp 170; GRP-170; Heat shock protein family H member 4; HSP 12A; Hypoxia up-regulated protein 1; Orp 150; ORP-150; oxygen regulated protein (150kD)
Gene Aliases: AI415631; Cab140; CBP-140; GRP-170; GRP170; HSP12A; HSPH4; HYOU1; ORP-150; ORP150
UniProt ID: (Human) Q9Y4L1, (Rat) Q63617, (Mouse) Q9JKR6
Entrez Gene ID: (Human) 10525, (Rat) 192235, (Mouse) 12282
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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