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Hepcidin 25 Antibody (BS-10348R) in IHC (P)
Paraformaldehyde-fixed, paraffin-embedded Human Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Hepcidin 25 Polyclonal Antibody, Unconjugated (bs-10348R) at 1:400 overnight at 4°C, followed by secondary antibody and DAB sta... View More
Product Details
BS-10348R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Hepcidin 25, amino acids 60-84.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hepcidin; LEAP-1; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor
Gene Aliases: HAMP; HEPC; HFE2B; LEAP1; PLTR; UNQ487/PRO1003
UniProt ID: (Human) P81172
Entrez Gene ID: (Human) 57817
negative regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
immune response
response to iron ion
killing of cells of other organism
defense response to bacterium
defense response to fungus
multicellular organismal iron ion homeostasis
negative regulation of intestinal absorption
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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