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            • Primary Antibodies ›
            • Histone H2A Antibodies

            Invitrogen

            Histone H2A Recombinant Rabbit Monoclonal Antibody (1H12)

            View all (30) Histone H2A antibodies
            Datasheet
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            Datasheet
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            Questions & Answers

            Cite Histone H2A Recombinant Rabbit Monoclonal Antibody (1H12)

            • Antibody Testing Data (2)
            Application
            Histone H2A Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            Histone H2A Antibody (MA5-33152) in ICC/IF

            Immunofluorescent analysis of Histone H2A in Hela cells using a Histone H2A monoclonal antibody (Product # MA5-33152) at a dilution of 1:168. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cell... View More
            View Product

            Histone H2A Antibody in Immunocytochemistry (ICC/IF)
            Histone H2A Antibody in Western Blot (WB)

            Product Details

            MA5-33152

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:5,000
            -

            Immunocytochemistry (ICC/IF)

            1:30-1:200
            -

            ELISA (ELISA)

            Assay-Dependent
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Expression System

            HEK293 cells

            Class

            Recombinant Monoclonal

            Type

            Antibody

            Clone

            1H12

            Immunogen

            Synthesized Histone H2A (20-50aa)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            2 mg/mL

            Purification

            Protein G, Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C or -80°C if preferred

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2811968

            Target Information

            Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: Acetyl-Histone H2; H2A 615 antibody; H2A GL101 antibody; H2A histone family member; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A histone family, member X; H2A histone family, member Z; H2A-clustered histone 1; H2A-clustered histone 12; H2A.1; H2A.1 antibody; H2A/d; H2a/x; H2A/z; h2ad; H2ae; h2ai; H2ak; H2AK5ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST1H2AE antibody; HIST3H2A; histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; Histone H2A type 1; Histone H2A type 1-A; Histone H2A type 1-B/E; Histone H2A type 1-D; Histone H2A type 1-H; Histone H2A type 1-J; Histone H2A.2; Histone H2A.3; Histone H2A.X; Histone H2A.Z; Histone H2A/a; Histone H2A/e; Histone H2A/g; Histone H2A/m; histone H2A/p; Histone H2A/ptl; Histone H2A/r; Histone H2A/s; histone H2AD; histone H2AE; Histone H2AX; Histone H2B type 1-A; Histone H2B, testis; MGC74460 antibody; Testis-specific histone H2B; TSH2B.1

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            Gene Aliases: bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AC1; H2AC11; H2AC12; H2AC13; H2AC14; H2AC15; H2AC16; H2AC17; H2AC4; H2AC7; H2AC8; H2AFA; H2AFALii; H2AFC; H2AFD; H2AFE; H2AFG; H2AFI; H2AFM; H2AFN; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AX; H2AZ; H2AZ1; H2BC1; H2BFU; HIST1H2AA; HIST1H2AB; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2AK; HIST1H2AL; HIST1H2AM; HIST1H2BA; pH2A/f; STBP; TH2A; TH2B; TSH2B; TSH2B.1

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            UniProt ID: (Human) P16104, (Human) P0C0S5, (Human) Q96QV6, (Human) P04908, (Human) P20671, (Human) P0C0S8, (Human) Q96KK5, (Human) Q99878, (Human) Q6NZA0

            View more View less

            Entrez Gene ID: (Human) 3014, (Human) 3015, (Human) 221613, (Human) 8335, (Human) 3013, (Human) 3012, (Human) 8969, (Human) 85235, (Human) 8329, (Human) 8331, (Human) 255626

            View more View less

            Function(s)

            DNA binding damaged DNA binding protein binding enzyme binding histone binding protein heterodimerization activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding chromatin DNA binding nucleosomal DNA binding molecular_function histone

            Process(es)

            DNA damage checkpoint double-strand break repair via homologous recombination double-strand break repair double-strand break repair via nonhomologous end joining nucleosome assembly chromatin silencing cellular response to DNA damage stimulus spermatogenesis response to ionizing radiation viral process cerebral cortex development positive regulation of DNA repair meiotic cell cycle cellular response to gamma radiation cellular senescence positive regulation of transcription from RNA polymerase II promoter cellular response to estradiol stimulus negative regulation of cell proliferation biological_process nucleosome disassembly inflammatory response plasminogen activation spermatogenesis, exchange of chromosomal proteins positive regulation of binding mononuclear cell migration
            It has to be done as per old AB suggested Products section.
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