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Histone H4ac (pan-acetyl) Polyclonal Antibody

6 References

View all (9) H4ac pan-acetyl (K5,K8,K12,K16) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Application

Histone H4ac (pan-acetyl) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Histone H4ac (pan-acetyl) Antibody (39925) in ICC/IF

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence. HeLa cells stained at 2 µg/mL with Histone H4 pan-acetyl antibody. Top panel: Histone H4 pan-acetyl antibody. Middle panel: DAPI. Bottom panel: merge.

Histone H4ac (pan-acetyl) Antibody in Western Blot (WB)

Histone H4ac (pan-acetyl) Antibody in ChIP Assay (ChIP)

Histone H4ac (pan-acetyl) Antibody in ChIP-Sequencing (ChIP-Seq)

Histone H4ac (pan-acetyl) Antibody in Dot Blot (DB)

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Product Details

39925

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:1,000

Immunocytochemistry (ICC/IF)

1-2 µg/mL

ChIP assay (ChIP)

5-10 µg

View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

5-10 µg

View 2 publications 2 publications

Dot blot (DB)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fungi, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).

Conjugate

Unconjugated

Form

Liquid

Concentration

See Datasheet

Purification

Protein A

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2687872

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Functional Roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hot Spots.

Molecular and cellular biology

Getun IV,Wu Z,Fallahi M,Ouizem S,Liu Q,Li W,Costi R,Roush WR,Cleveland JL,Bois PRJ

Research topic: Cell Cycle, Meiosis

Wed Feb 01 00:00:00 UTC 2017

Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation.

Scientific reports

Flodby P,Li C,Liu Y,Wang H,Rieger ME,Minoo P,Crandall ED,Ann DK,Borok Z,Zhou B

Research topic: Lung Cancer

Wed Jun 14 00:00:00 UTC 2017

Using Chromatin Immunoprecipitation in Toxicology: A Step-by-Step Guide to Increasing Efficiency, Reducing Variability, and Expanding Applications.

Current protocols in toxicology

McCullough SD,On DM,Bowers EC

Research topic: Methods Paper

Tue May 02 00:00:00 UTC 2017

Loss of NR2E3 represses AHR by LSD1 reprogramming, is associated with poor prognosis in liver cancer.

Scientific reports

Khanal T,Choi K,Leung YK,Wang J,Kim D,Janakiram V,Cho SG,Puga A,Ho SM,Kim K

Research topic: Liver

Wed Sep 06 00:00:00 UTC 2017

IL-33 promotes MHC class II expression in murine mast cells.

Immunity, inflammation and disease

Ito T,Egusa C,Maeda T,Numata T,Nakano N,Nishiyama C,Tsuboi R

Research topic: Atopic Dermatitis

Tue Sep 01 00:00:00 UTC 2015

Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production.

Molecular microbiology

Soukup AA,Chiang YM,Bok JW,Reyes-Dominguez Y,Oakley BR,Wang CC,Strauss J,Keller NP

Research topic: Transcription Regulation

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: acetyl Histone H4; Acetyl-H4 K5 K8 K12 K16; Acetyl-H4 Lys5 Lys8 Lys12 Lys16; Acetyl-Histone H4 K5 K8 K12 K16; Acetyl-Histone H4 Lys5 Lys8 Lys12 Lys16; H4 ac pan-acetyl; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4ac; H4K12; H4K12a; H4K12ac; H4K16; H4K16ac; H4K5; H4K5,8,12,16ac; H4K5ac; H4K8; H4K8ac; H4Lys5,8,12,16ac; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K12; Histone H4K16; Histone H4K5; Histone H4K8; histone IV, family 2

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Gene Aliases: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652

UniProt ID: (Human) P62805, (Mouse) P62806, (Mouse) A4QMY0

Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504, (Mouse) 326619, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122

Function(s)

DNA binding protein binding protein domain specific binding histone binding poly(A) RNA binding protein heterodimerization activity histone

Process(es)

chromatin silencing at rDNA double-strand break repair via nonhomologous end joining nucleosome assembly DNA replication-dependent nucleosome assembly DNA replication-independent nucleosome assembly DNA-templated transcription, initiation telomere capping gene silencing by RNA telomere organization CENP-A containing nucleosome assembly cellular protein metabolic process negative regulation of megakaryocyte differentiation negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization beta-catenin-TCF complex assembly

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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