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Invitrogen
ICSP Polyclonal Antibody
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Application
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ICSP Antibody (PA5-144411) in WB
Western blot analysis of ICSP in recombinant protein using a dilution of 1:2,000. Samples were incubated with ICSP polyclonal antibody (Product # PA5-144411). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 37.4 kDa. Observed band size: 39 kDa.
Product Details
PA5-144411
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Shigella flexneri ICSP protein (21-315aa)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.87 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091655
Target Information
Protease responsible for the cleavage of IcsA between 'Arg-758' and 'Arg-759', removing the entire alpha domain from IscA localized on the bacterial surface. This proteolytic activity contributes to the maintenance of a tight polar cap of IcsA, which is important to Shigella actin-based motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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