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Product Details
PA5-100197
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ID3(Accession Q02535), corresponding to amino acid residues S65-R115.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815727
Product Specific Information
Antibody detects endogenous levels of total ID3.
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Gene Aliases: 1R21; BHLHB25; HEIR-1; HEIR1; Hlh462; Id-3; ID3; Idb3
UniProt ID: (Human) Q02535, (Rat) P41138, (Mouse) P41133
Entrez Gene ID: (Human) 3399, (Rat) 25585, (Mouse) 15903
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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