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Product Details
CF500757
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI15E3
Immunogen
Full length human recombinant protein of human ID3 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID-like protein inhibitor HLH 1R21; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Gene Aliases: 1R21; BHLHB25; HEIR-1; HEIR1; ID3
UniProt ID: (Human) Q02535
Entrez Gene ID: (Human) 3399
negative regulation of transcription from RNA polymerase II promoter
metanephros development
regulation of DNA replication
transcription, DNA-templated
multicellular organism development
central nervous system development
heart development
muscle organ development
circadian rhythm
response to wounding
neuron differentiation
epithelial cell differentiation
notochord development
odontogenesis
positive regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of myoblast differentiation
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
regulation of cell cycle
cellular response to leptomycin B
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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