Invitrogen

IFN gamma Monoclonal Antibody (350B 10G6)

FIGURE: 1 / 5

IFN gamma Antibody (AHC4432) in ELISA

Nature communications 2017 - Fig. 1 Binding of human glycosylated cytokines to galectin-3-coated beads. Ratio refers to molar ratio. a hIFNgamma measured by ELISA in the supernatant after incubation with galectin-3-coated beads in the presence or absence of 100 mM lactose. The glycosylated IFNgamma was produced in CHO cells and the unglycosylated IFNgamma was produced in E. coli . Mean +- SD of one representative experiment of 8 (glyco... View More

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Product Details

AHC4432

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

350B 10G6

Immunogen

Purified recombinant human interferon-gamma (IFN-gamma).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.5mg/mL sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536280

Product Specific Information

This product is recommended as a capture antibody in Sandwich ELISA applications.

This clone can also be referred to as clone number 350B 10G6 R3.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Galectin-3 captures interferon-gamma in the tumor matrix reducing chemokine gradient production and T-cell tumor infiltration.

Nature communications

Gordon-Alonso M,Hirsch T,Wildmann C,van der Bruggen P

Published figure using IFN gamma monoclonal antibody (Product # AHC4432) in ELISA

Fri Oct 06 00:00:00 EDT 2017

A Homogeneous Immunoassay Method for Detecting Interferon-Gamma in Patients with Latent Tuberculosis Infection.

Journal of microbiology and biotechnology

Wu F,Wang L,Guo Q,Zhao M,Gu H,Xu H,Lou J

AHC4432 was used to utilize a homogeneous immunoassay method to identify interferon-gamma in patients with latent tuberculosis infection

Tue Mar 01 00:00:00 EST 2016

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

Immunology letters

Chen ML,Tsai TC,Lin YY,Tsai YM,Wang LK,Lee MC,Tsai FM

AHC4432 was used in blocking assay to examine how antipsychotic drugs affect immune responses.

Sun Oct 30 00:00:00 EDT 2011

Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses.

PloS one

Daubersies P,Ollomo B,Sauzet JP,Brahimi K,Perlaza BL,Eling W,Moukana H,Rouquet P,de Taisne C,Druilhe P

AHC4432 was used in ELISA to discuss the hurdles involved in human genetic immunization

Wed Jul 16 00:00:00 EDT 2008

Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota.

Clinical and experimental immunology

Takeda K,Suzuki T,Shimada SI,Shida K,Nanno M,Okumura K

AHC4432 was used in ELISA to evaluate the effect of Lactobacillus casei Shirota on human natural killer cell activity

Sun Oct 01 00:00:00 EDT 2006

The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.

Experimental gerontology

Njemini R,Lambert M,Demanet C,Mets T

AHC4432 was used in ELISA to examine the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells

Wed Mar 01 00:00:00 EST 2006

Impaired interleukin (IL)-4-associated generation of CCR4-expressing T cells in neonates with hereditary allergy risk.

Clinical and experimental immunology

Haddeland U,Sletten GB,Brandtzaeg P,Nakstad B

AHC4432 was used in ELISA to investigate how early antigen encounters may influence allergy

Tue Feb 01 00:00:00 EST 2005

Methylation-dependent T cell immunity to Mycobacterium tuberculosis heparin-binding hemagglutinin.

Nature medicine

Temmerman S,Pethe K,Parra M,Alonso S,Rouanet C,Pickett T,Drowart A,Debrie AS,Delogu G,Menozzi FD,Sergheraert C,Brennan MJ,Mascart F,Locht C

AHC4432 was used in ELISA to report that methylation of the Mycobacterium tuberculosis heparin-binding hemagglutinin is essential for effective T cell immunity to this antigen

Wed Sep 01 00:00:00 EDT 2004

Oxidative stress as a trigger for cellular immune responses in patients with alcoholic liver disease.

Hepatology (Baltimore, Md.)

Stewart SF,Vidali M,Day CP,Albano E,Jones DE

AHC4432 was used in ELISA to investigate antibodies produced by patients with alcoholic liver disease

Thu Jan 01 00:00:00 EST 2004

Identification of a conserved region of Plasmodium falciparum MSP3 targeted by biologically active antibodies to improve vaccine design.

The Journal of infectious diseases

Singh S,Soe S,Mejia JP,Roussilhon C,Theisen M,Corradin G,Druilhe P

AHC4432 was used in ELISA to investigate epitopes of merozoite surface protein 3

Wed Sep 01 00:00:00 EDT 2004

Insulin-like growth factor-I stimulates IL-10 production in human T cells.

Journal of leukocyte biology

Kooijman R,Coppens A

AHC4432 was used in ELISA to explore the immunomodulatory effects of insulin-like growth factor through regulation of cytokine production

Fri Oct 01 00:00:00 EDT 2004

Clinical cancer research : an official journal of the American Association for Cancer Research

Messmann RA,Ullmann CD,Lahusen T,Kalehua A,Wasfy J,Melillo G,Ding I,Headlee D,Figg WD,Sausville EA,Senderowicz AM

Sat Feb 01 00:00:00 EST 2003

Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Mascart F,Verscheure V,Malfroot A,Hainaut M,Piérard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C

Sat Feb 01 00:00:00 EST 2003

Optimal kinetics for quantification of antigen-induced cytokines in human peripheral blood mononuclear cells by real-time PCR and by ELISA.

Journal of immunological methods

Listvanova S,Temmerman S,Stordeur P,Verscheure V,Place S,Zhou L,Locht C,Mascart F

AHC4432 was used in ELISA to use RT-PCR for the quantification of antigen-induced cytokine mRNA.

Wed Oct 01 00:00:00 EDT 2003

A novel ELISPOT assay to enhance detection of antigen-specific T cells employing antigen-presenting cells expressing vector-driven human B7-1.

Journal of immunological methods

Arlen PM,Gulley JL,Palena C,Marshall J,Schlom J,Tsang KY

Fri Aug 01 00:00:00 EDT 2003

Biological characterization of lipopolysaccharide from Treponema pectinovorum.

Infection and immunity

Kesavalu L,Falk CW,Davis KJ,Steffen MJ,Xu X,Holt SC,Ebersole JL

Tue Jan 01 00:00:00 EST 2002

Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.

International immunology

Bullens DM,Rafiq K,Charitidou L,Peng X,Kasran A,Warmerdam PA,Van Gool SW,Ceuppens JL

AHC4432 was used in ELISA to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines.

Thu Feb 01 00:00:00 EST 2001

Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.

Infection and immunity

Nixon CS,Steffen MJ,Ebersole JL

Fri Sep 01 00:00:00 EDT 2000

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC4432 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC4432 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Activated natural killer cell tumor retention and cytokine production in colon tumor using a tissue-isolated model.

The Journal of surgical research

Ribeiro U,Whiteside TL,Basse PH,Safatle-Ribeiro AV,Huneke CE,Posner MC

AHC4432 was used in immunohistochemistry - frozen section to study intratumor activated natural killer retention and cytokine production

Mon Mar 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)

cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (350B 10G6)

IFN gamma Antibody (AHC4432) in ELISA

Product Details

AHC4432

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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