Invitrogen

IFN gamma Monoclonal Antibody (R4-6A2), Biotin, Functional Grade, eBioscience™

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Published Figures (3)

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IFN gamma Antibody (36-7312-85) in ELISA

JCI insight 2018 - Figure 1 Acox1 Lampe1 mutation promotes proinflammatory immune responsiveness. (A and B) Twelve-week-old, chow diet-fed, Acox1 Lampe1 mice and WT littermate controls were challenged i.p. with biotinylated capture Ab (20 mug/mouse) 3 hours prior to i.v. saline (NS) or LPS challenge (25 mug/mouse; ultrapure E. coli 0111:B4). Twenty-four hours later, serum was collected, and IL-6, TNF-alpha, IFN-gamma, and IL-... View More

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Product Details

36-7312-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 4 publications 4 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

R4-6A2

Conjugate

Biotin , Functional Grade Biotin , Functional Grade Biotin , Functional Grade

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469764

Product Specific Information

Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The Functional Grade Biotin anti-mouse IFNg (R4-6A2) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.

Applications Tested: The Functional Grade Biotin R4-6A2 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the purified AN-18 (14-7313) antibody for capture and recombinant mouse IFNg (39-8311) as the standard.A suitable range of concentrations of the FG Biotin R4-6A2 antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/mL - 1.6 pg/mL (AN-18 assay) should be included in each ELISA plate.

Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

36-7312 was used in Flow cytometry/Cell sorting to suggest that miR-21-5p may protect melanocytes via targeting STAT3 and regulating the regulatory T cell/effector T cell balance, providing a possible treatment for managing vitiligo.

Fri Jan 01 00:00:00 EST 2021

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Fri Jan 01 00:00:00 EST 2021

Fine-tuning of antigen-specific immune responses by regulatory T cells activated via antigen recognition-independent and humoral factor-dependent mechanisms.

Scandinavian journal of immunology

Kojima H,Kashiwakura Y,Kanno Y,Hashiguchi M,Kobata T

36-7312-85 was used in Functional Assay, ELISA to suggest that Tregs are activated by cytokines, mainly IL-2, released from T cells that are activated by a specific antigen.

Tue Jun 01 00:00:00 EDT 2021

Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8⁺ T cells.

Scientific reports

Miranda-Katz M,Erickson JJ,Lan J,Ecker A,Zhang Y,Joyce S,Williams JV

Published figure using IFN gamma monoclonal antibody (Product # 36-7312-85) in ELISA

Thu Oct 21 00:00:00 EDT 2021

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

36-7312 was used in Functional assays to demonstrate that β-oxidation links metabolism and immune responsiveness.

Thu Mar 22 00:00:00 EDT 2018

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

36-7312 was used in Functional assays to demonstrate that β-oxidation links metabolism and immune responsiveness.

Thu Mar 22 00:00:00 EDT 2018

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Journal of neuroimmunology

Kort JJ,Kawamura K,Fugger L,Weissert R,Forsthuber TG

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (R4-6A2), Biotin, Functional Grade, eBioscience™

IFN gamma Antibody (36-7312-85) in ELISA

Product Details

36-7312-85

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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