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Application
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IGFBP2 Antibody (702196) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of T-47D (Lane 1), T-47D (Condition Media) (Lane 2), PC-3 (Lane 3), PC-3 (Condition Media) (Lane 4), A549 (Lane 5) and A549 (Condition Media) (Lane 6). The blots were probed with Anti-IGFBP2 Recombinant Rabbit Monoclonal Antibody (Product # 702196, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 50 kDa Glycosylated and 35 kDa band for... View More
Product Details
702196
Product Specifications
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H2L9
Immunogen
Protein corresponding to Human IGFBP2 (aa 130-325)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633066
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, Sheep and Goat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IGFBP2 (insulin-like growth factor binding protein 2) is a member of the ISGBP family which bind various IGFs. IGFBP2 is overexpressed in a wide spectrum of cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The overexpression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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