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IL-15 Antibody (MA5-23663) in Flow
Flow cytometry of IL-15 in Human peripheral blood monocytes, resting (open histogram), or treated with 1 μg/mL LPS overnight (filled histogram). Samples were incubated in IL-15 monoclonal antibody (Product # MA5-23663) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
Product Details
MA5-23663
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
34559
Immunogen
E. coli-derived recombinant human IL-15 Asn49-Ser162
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608839
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
Target Information
IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: il 15; IL-15; ILN; Interleukin; Interleukin-15; Interleukin15; M-IL-15; MGC9721
Gene Aliases: IL-15; IL15
UniProt ID: (Human) P40933
Entrez Gene ID: (Human) 3600
natural killer cell differentiation
NK T cell proliferation
inflammatory response
immune response
signal transduction
cell-cell signaling
aging
positive regulation of cell proliferation
skeletal muscle atrophy
hyaluronan metabolic process
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of natural killer cell differentiation
positive regulation of tissue remodeling
positive regulation of T cell proliferation
tyrosine phosphorylation of Stat5 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
extrathymic T cell selection
regulation of T cell differentiation
cell maturation
lymph node development
negative regulation of smooth muscle cell proliferation
regulation of defense response to virus by host
positive regulation of inflammatory response
positive regulation of immune response
cellular response to vitamin D
positive regulation of protein O-linked glycosylation
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