Invitrogen

IL-8 (CXCL8) Monoclonal Antibody (I8-S2), Biotin

8 Published Figures

17 References

View all (72) IL-8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

IL-8 (CXCL8) Antibody (M802B) in ELISA

Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 µL per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/mL across a 3 µg/mL mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 µL per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 µg/mL for 1 hour ... View More

IL-8 (CXCL8) Antibody in Radioimmune assays (RIA)

Product Image Gallery

Product Details

M802B

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 16 publications 16 publications

Radioimmune Assays (RIA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

I8-S2

Immunogen

Recombinant human IL-8

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223584

Product Specific Information

M802B targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M802B immunogen is recombinant human IL-8.

M802B detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M802B IL8 antibody (biotinylated conjugate of clone I8-S2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M801 (clone 3IL8-H10). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M802B (clone I8-S2) and antibody M801 (clone 3IL8-H10) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic effects of two rutile TiO₂ nanomaterials in human intestinal and hepatic cell lines.

Particle and fibre toxicology

Jalili P,Krause BC,Lanceleur R,Burel A,Jungnickel H,Lampen A,Laux P,Luch A,Fessard V,Hogeveen K

M802B was used in Enzyme-linked immunosorbent assay to indicate that although no clear effects on cytotoxicity were observed following repeated exposure of differentiated Caco-2 and HepaRG cells to TiO2 NMs, subtle effects on membrane composition could induce potential adverse effects in the long-term.

Tue May 17 00:00:00 EDT 2022

Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells.

Journal of periodontology

Kantrong N,Chang AM,Bamashmous S,Hajjar AM,Bucala RJ,Darveau RP

M802B was used in Enzyme-linked immunosorbent assay to demonstrate that MIF is a significant contributor to the maintenance of healthy oral homeostasis.

Thu Dec 01 00:00:00 EST 2022

Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin.

Scientific reports

Seiser S,Janker L,Zila N,Mildner M,Rakita A,Matiasek J,Bileck A,Gerner C,Paulitschke V,Elbe-Bürger A

M802B was used in Enzyme-linked immunosorbent assay to give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.

Fri Jan 08 00:00:00 EST 2021

Combined effects of okadaic acid and pectenotoxin-2, 13-desmethylspirolide C or yessotoxin in human intestinal Caco-2 cells.

Chemosphere

Alarcan J,Barbé S,Kopp B,Hessel-Pras S,Braeuning A,Lampen A,Le Hégarat L,Fessard V

M802B was used in ELISA to examine the effects of three binary mixtures, OA/PTX-2, OA/SPX-1 and OA/YTX on human intestinal Caco-2 cells.

Thu Aug 01 00:00:00 EDT 2019

Inflammatory cytokines tumor necrosis factor-α, interleukin-8 and sleep monitoring in patients with obstructive sleep apnea syndrome.

Experimental and therapeutic medicine

Ming H,Tian A,Liu B,Hu Y,Liu C,Chen R,Cheng L

M802B was used in an ELISA assay to investigate the changes of tumour necrosis factor-α, interleukin-8 and sleep ability in patients with obstructive sleep apnea hypopnea syndrome.

Fri Mar 01 00:00:00 EST 2019

Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Croasdell A,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Thu Sep 01 00:00:00 EDT 2016

The human salivary proteome is radiation responsive.

Radiation research

Moore HD,Ivey RG,Voytovich UJ,Lin C,Stirewalt DL,Pogosova-Agadjanyan EL,Paulovich AG

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in Radioimmune Assays

Thu May 01 00:00:00 EDT 2014

Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.

PloS one

Sahler J,Woeller CF,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Mon Jul 13 00:00:00 EDT 2015

Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.

American journal of physiology. Lung cellular and molecular physiology

McMillan DH,Woeller CF,Thatcher TH,Spinelli SL,Maggirwar SB,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Sat Jun 01 00:00:00 EDT 2013

Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

British journal of pharmacology

Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG

M802B was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells

Wed Aug 01 00:00:00 EDT 2012

Optical protein sensor for detecting cancer markers in saliva.

Biosensors & bioelectronics

Tan W,Sabet L,Li Y,Yu T,Klokkevold PR,Wong DT,Ho CM

M802B was used in ELISA to develop a new sensor for cancer detection in saliva

Wed Oct 15 00:00:00 EDT 2008

Optical protein sensor for detecting cancer markers in saliva.

Biosensors & bioelectronics

Tan W,Sabet L,Li Y,Yu T,Klokkevold PR,Wong DT,Ho CM

M802B was used in ELISA to develop a new sensor for cancer detection in saliva

Wed Oct 15 00:00:00 EDT 2008

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M802B was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 EST 2007

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M802B was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 EDT 2006

Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.

British journal of pharmacology

Beigi RD,Kertesy SB,Aquilina G,Dubyak GR

M802B was used in ELISA to study the role of oxidized ATP in proinflammatory signaling

Wed Oct 01 00:00:00 EDT 2003

Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Cell biology and toxicology

Urbanowska T,Mangialaio S,Hartmann C,Legay F

M802B was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease

Sun Jun 01 00:00:00 EDT 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M802B was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 EDT 2003

Regulation of interleukin 8 expression in human malignant melanoma cells.

Cancer research

Singh RK,Varney ML

M802B was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells

Wed Apr 01 00:00:00 EST 1998

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

View more View less

Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

View more View less

UniProt ID: (Human) P10145

View more View less

Entrez Gene ID: (Human) 3576

View more View less

Function(s)

interleukin-8 receptor binding protein binding chemokine activity chemokine

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-8 (CXCL8) Monoclonal Antibody (I8-S2), Biotin

IL-8 (CXCL8) Antibody (M802B) in ELISA

Product Details

M802B

Western Blot (WB)

ELISA (ELISA)

Radioimmune Assays (RIA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!