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            • Primary Antibodies ›
            • IL36B Antibodies

            Invitrogen

            IL36B Polyclonal Antibody

            View all (15) IL36B antibodies
            Datasheet
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            Catalog # PA5-100833
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            Prix (EUR)
            493,00
            100 µL
             
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            Cite IL36B Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            IL36B Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            IL36B Antibody (PA5-100833) in ICC/IF

            Immunocytochemistry analysis of IL36B in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with IL36B polyclonal antibody (Product # PA5-100833) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
            View Product

            IL36B Antibody in Immunocytochemistry (ICC/IF)
            IL36B Antibody in Western Blot (WB)

            Product Details

            PA5-100833

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            -

            Immunocytochemistry (ICC/IF)

            1:100-1:500
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthesized peptide derived from human IL36B(Accession Q9NZH7), corresponding to amino acid residues W126-M164.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2850334

            Product Specific Information

            Antibody detects endogenous levels of total IL1F8.

            Target Information

            IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP. Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown. The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: family of interleukin 1-eta; FIL1 eta; IL-1 eta; IL-1F8; IL-1F8 (FIL1-eta); IL-1H2; IL1F8 (Canonical product IL-1F8a); Interleukin; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 beta

            View more View less

            Gene Aliases: 2310043N20Rik; FIL1; FIL1-(ETA); Fil1e; FIL1H; FILI-(ETA); IL-1F8; IL-1H2; IL1-ETA; IL1F8; IL1H2; IL36B

            View more View less

            UniProt ID: (Human) Q9NZH7, (Mouse) Q9D6Z6

            View more View less

            Entrez Gene ID: (Human) 27177, (Mouse) 69677

            View more View less

            Function(s)

            cytokine activity interleukin-1 receptor binding interleukin superfamily

            Process(es)

            inflammatory response immune response cytokine-mediated signaling pathway positive regulation of interleukin-6 production innate immune response positive regulation of T cell differentiation positive regulation of cytokine production immune system process
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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