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Invitrogen

INSR alpha Monoclonal Antibody (83-14)

Advanced Verification

5 References

View all (15) INSR alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

Application

INSR alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

INSR alpha Antibody (MA5-13759) in ICC/IF

Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the membrane of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product ... View More

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Product Details

MA5-13759

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

2 µg/mL

View 3 publications 3 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

83-14

Immunogen

IM-9 lymphocytes followed by purified insulin receptor

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985953

Product Specific Information

MA5-13759 targets Insulin Receptor alpha in WB, ICC/IF, ELISA and IP applications and shows reactivity with Human samples.

The MA5-13759 immunogen is iM-9 lymphocytes followed by purified insulin receptor.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression.

Cell

Hancock ML,Meyer RC,Mistry M,Khetani RS,Wagschal A,Shin T,Ho Sui SJ,Näär AM,Flanagan JG

MA5-13759 was used in Immunocytochemistry to show that IR associates with RNA polymerase II in the nucleus, with striking enrichment at promoters genome-wide.

Thu Apr 18 00:00:00 UTC 2019

Inhibition of death-receptor mediated apoptosis in human adipocytes by the insulin-like growth factor I (IGF-I)/IGF-I receptor autocrine circuit.

Endocrinology

Fischer-Posovszky P,Tornqvist H,Debatin KM,Wabitsch M

MA5-13759 was used in immunoprecipitation to study the role of the IGF-1/IGF-1R autocrine circuit in inhibition of death-receptor mediated apoptosis in human adipocytes

Thu Apr 01 00:00:00 UTC 2004

Glucosamine induces resistance to insulin-like growth factor I (IGF-I) and insulin in Hep G2 cell cultures: biological significance of IGF-I/insulin hybrid receptors.

Endocrinology

Sakai K,Clemmons DR

MA5-13759 was used in blocking or activating experiment to investigate the influence of glucosamine on Hep G2 cell sensitivity to IGF-I and insulin

Sun Jun 01 00:00:00 UTC 2003

An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events.

Diabetologia

Hamer I,Foti M,Emkey R,Cordier-Bussat M,Philippe J,De Meyts P,Maeder C,Kahn CR,Carpentier JL

MA5-13759 was used in immunoprecipitation to study the function of a mutant insulin receptor from a patient with severe insulin resistance

Wed May 01 00:00:00 UTC 2002

Increases in free, unbound insulin-like growth factor I enhance insulin responsiveness in human hepatoma G2 cells in culture.

The Journal of biological chemistry

Sakai K,Lowman HB,Clemmons DR

MA5-13759 was used in immunoprecipitation to investigate the effect of a synthetic peptide bp1-01 on the binding of IGF-I to IGFBP-1 and insulin responsiveness in HepG2 cells

Fri Apr 19 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: CD220; CD220 beta; Insulin receptor; IR; IR alpha; IR beta

Gene Aliases: CD220; HHF5; INSR

UniProt ID: (Human) P06213

Entrez Gene ID: (Human) 3643

Function(s)

protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding transmembrane signal receptor

Process(es)

Performance Guarantee

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