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Invitrogen

IRAK1 Polyclonal Antibody

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4 Published Figures

4 References

View all (33) IRAK1 antibodies

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Catalog # PA5-19855

Precio (EUR)

522,00

100 µg

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Datasheet

Protocols

Questions & Answers

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Application

FIGURE: 1 / 6

IRAK1 Antibody (PA5-19855) in WB

Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-19855) and an 80 kDa band corresponding to IRAK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), K-562 (Lane 5), U-2 OS (Lane 6) and T-47D (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB2100... View More

IRAK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

IRAK1 Antibody — Figure 5 Analysis of E2 signal transduction. ( a ) Western blotting of A549, MCF-10A, and 293T cell lysates showing IL-1R expression; cytoplasmic and membrane components of the cell lysates were separated. A549 cells were included as a positive control for IL-1R1 expression, and 293T cells were used as a negative control. ( b ) Western blotting of MCF-10A cells showing phospho-IRAK1 (left) and IRAK1 (right) following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( c ) Western blotting of MCF-10A cells probed to examine phospho-p38(Thr180/Tyr182) and p38 after adding 100 ng/ml GST-IL-1beta for 0-60 min. ( d ) Western blotting of MCF-10A cells probed to examine phospho-p38 (Thr180/Tyr182) and p38 after adding 1 ng/ml GST-IL-1beta for 0-60 min. ( e ) Western blotting of MCF-10A cells showing phospho-JNK(Thr183/Tyr185) and JNK following treatment with 100 ng/ml GST-IL-1beta for 0-60 min. ( f ) Western blotting of MCF-10A cells showing phospho-IkB (Ser32) and IkB following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( g ) MMP-3 activity assay of MCF-10A cells following treatment with 100 ng/ml GST-IL-1beta with or without 1.6 muM NNGH, which was used as an MMP-3 inhibitor. Four independent experiments were performed. Bars represent +/-SD. ( h ) Representative confocal images of MCF-10A cells in a 3D culture treated with 100 ng/ml GST-IL-1beta (second row) or E2 (32 nM, third row) or control (first row) for 7 days to examine the basement membrane via immunofluorescenc {TM}

Product Details

PA5-19855

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

Immunoprecipitation (IP)

2-4 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to amino acids near the carboxy terminus of human IRAK.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152551

Product Specific Information

A suggested positive control is Hela cell lysate.

PA5-19855 can be used with blocking peptide PEP-0001.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

PA5-19855 was used in Western Blotting and Immunoprecipitaiton to suggest a potential target for the novel therapeutic approaches of multiple sclerosis (MS)/EAE and NLRP3-related inflammatory diseases.

Fri Feb 10 00:00:00 UTC 2023

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

Fri Feb 10 00:00:00 UTC 2023

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

Fri Feb 10 00:00:00 UTC 2023

Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1.

Theranostics

Li X,Liao J,Su X,Li W,Bi Z,Wang J,Su Q,Huang H,Wei Y,Gao Y,Li J,Liu L,Wang C

PA5-19855 was used in Western Blotting to study the protective effects of human urine-derived stem cells against a rat model of renal ischemia/reperfusion injury.

Tue Jun 01 00:00:00 UTC 2021

Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1.

Theranostics

Li X,Liao J,Su X,Li W,Bi Z,Wang J,Su Q,Huang H,Wei Y,Gao Y,Li J,Liu L,Wang C

PA5-19855 was used in Western Blotting to study the protective effects of human urine-derived stem cells against a rat model of renal ischemia/reperfusion injury.

Tue Jun 01 00:00:00 UTC 2021

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Scientific reports

Deng Y,Miki Y,Nakanishi A

Published figure using IRAK1 polyclonal antibody (Product # PA5-19855) in Western Blot

Tue Jan 28 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase

Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk; RGD1563841

UniProt ID: (Human) P51617, (Mouse) Q62406

Entrez Gene ID: (Human) 3654, (Mouse) 16179, (Rat) 363520

Function(s)

protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding kinase activity heat shock protein binding protein homodimerization activity protein heterodimerization activity nucleotide binding interleukin-1 receptor binding transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation signal transduction transmembrane receptor protein serine/threonine kinase signaling pathway activation of NF-kappaB-inducing kinase activity JNK cascade aging lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity innate immune response positive regulation of transcription, DNA-templated protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity protein oligomerization nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to hypoxia immune system process phosphorylation cytokine-mediated signaling pathway response to peptidoglycan intracellular signal transduction positive regulation of JUN kinase activity negative regulation of transcription, DNA-templated negative regulation of cholesterol efflux cellular response to lipopolysaccharide

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRAK1 Polyclonal Antibody

IRAK1 Antibody (PA5-19855) in WB

Product Details

PA5-19855

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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IRAK1 Polyclonal Antibody

IRAK1 Antibody (PA5-19855) in WB

Product Details

PA5-19855

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Related Products for

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!