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IRF1 Monoclonal Antibody (2E4)

View all (22) IRF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

FIGURE: 1 / 1

IRF1 Antibody (H00003659-M01) in WB

IRF1 monoclonal antibody (M01), clone 2E4. Western Blot analysis of IRF1 expression in COLO 320 HSR.

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Product Details

H00003659-M01

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

ELISA (ELISA)

10 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2E4

Immunogen

IRF1 (NP_002189, 216 a.a. approximately 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Product may be used with Western Blot (Cell lysate).

Immunogen sequence: PMPSTSEATT DEDEEGKLPE DIMKLLEQSE WQPTNVDGKG YLLNEPGVQP TSVYGDFSCK EEPEIDSPGG DIGLSLQRVF TDLKNMDATW LDSLLTPVRL PSIQAIPCAP

Target Information

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppression. Diseases associated with IRF1 include gastric cancer, somatic and lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Interferon regulatory factor 1; interferon regulatory factor 1 isoform +I9; interferon regulatory factor 1 isoform d78; interferon regulatory factor 1 isoform delta4; interferon regulatory factor 1 isoform delta7; IRF-1

Gene Aliases: IRF-1; IRF1; MAR

UniProt ID: (Human) P10914

Entrez Gene ID: (Human) 3659

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding winged helix/forkhead transcription factor

Process(es)

regulation of adaptive immune response transcription from RNA polymerase II promoter apoptotic process cell cycle arrest blood coagulation negative regulation of cell proliferation positive regulation of type I interferon production positive regulation of interferon-beta production regulation of MyD88-dependent toll-like receptor signaling pathway cellular response to interferon-beta CD8-positive, alpha-beta T cell differentiation positive regulation of interleukin-12 biosynthetic process regulation of innate immune response negative regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter defense response to virus regulation of cell cycle interferon-gamma-mediated signaling pathway type I interferon signaling pathway cellular response to mechanical stimulus regulation of CD8-positive, alpha-beta T cell proliferation

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