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Invitrogen
IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™
Application
FIGURE: 1 / 4
IRF7 Antibody (12-5829-82) in Flow
Mouse splenocytes were untreated (right, orange histogram) or treated with CpG ODN 2395 (right, purple histogram) for 5 hours. Using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, the cells were then intracellularly stained with Anti-Mouse CD317 (BST2, PDCA-1) FITC (Product # 11-3172-82), Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82), and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (right, blue histogram) or 0.125 µg of Anti-Mouse IRF7 PE. Viable CD317+... View More
Product Details
12-5829-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MNGPKL
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572629
Product Specific Information
Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events causeIRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription.
Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 7; IRF-7
Gene Aliases: Irf7
UniProt ID: (Mouse) P70434
Entrez Gene ID: (Mouse) 54123
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