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Invitrogen
IRF8 Monoclonal Antibody (ZI003)
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Application
FIGURE: 1 / 7
IRF8 Antibody (39-8800) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), K-562 (Lane 3), HL-60 (Lane 4), U-2 OS (Lane 5),THP-1 (Lane 6), NIH/3T3 (Lane 7) and T98G (Lane 8). The blot was probed with Anti- IRF8 Mouse Monoclonal Antibody (Product # 39-8800, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to IRF8 was observed in Raji, Ramos and not observ... View More
Product Details
39-8800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ZI003
Immunogen
Synthetic peptide derived from the C-terminal region of the human ICSBP protein
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533439
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8
Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls
UniProt ID: (Human) Q02556, (Mouse) P23611
Entrez Gene ID: (Human) 3394, (Mouse) 15900
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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