Proteintech

IRS1 Polyclonal Antibody

IRS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

IRS1 Antibody (17509-1-AP) in ICC/IF

Immunofluorescent analysis of A549 cells using 17509-1-AP (IRS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IRS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IRS1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17509-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 44 publications 44 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fish, Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IRS1 Fusion Protein Ag11714 (895-1243 aa encoded by BC053895)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EYVNIEFGS DQSGYLSGPV AFHSSPSVRC PSQLQPAPRE EETGTEEYMK MDLGPGRRAA WQESTGVEMG RLGPAPPGAA SICRPTRAVP SSRGDYMTMQ MSCPRQSYVD TSPAAPVSYA DMRTGIAAEE VSLPRATMAA ASSSSAASAS PTGPQGAAEL AAHSSLLGGP QGPGGMSAFT RVNLSPNRNQ SAKVIRADPQ GCRRRHSSET FSSTPSATRV GNTVPFGAGA AVGGGGGSSS SSEDVKRHSS ASFENVWLRP GELGGAPKEP AKLCGAAGGL ENGLNYIDLD LVKDFKQCPQ ECTPEPQPPP PPPPHQPLGS GESSSTRRSS EDLSAYASIS FQKQPEDRQ (895-1243 aa encoded by BC053895)

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Visceral adipose tissue-directed human kallistatin gene therapy improves adipose tissue remodeling and metabolic health in obese mice.

Cellular signalling

Wu Y,Jiang Y,Guo JQ,Yang ZW,Carvalho A,Qian LL,Ji JJ,Ji ZJ,Ma GS,Yao YY

Thu Jun 01 00:00:00 EDT 2023

Icariin ameliorates memory deficits through regulating brain insulin signaling and glucose transporters in 3×Tg-AD mice.

Neural regeneration research

Yan F,Liu J,Chen MX,Zhang Y,Wei SJ,Jin H,Nie J,Fu XL,Shi JS,Zhou SY,Jin F

Sun Jan 01 00:00:00 EST 2023

Overexpression of Insulin Receptor Substrate 1 (IRS1) Relates to Poor Prognosis and Promotes Proliferation, Stemness, Migration, and Oxidative Stress Resistance in Cholangiocarcinoma.

International journal of molecular sciences

Kaewlert W,Sakonsinsiri C,Lert-Itthiporn W,Ungarreevittaya P,Pairojkul C,Pinlaor S,Murata M,Thanan R

Thu Jan 26 00:00:00 EST 2023

Overexpression of Insulin Receptor Substrate 1 (IRS1) Relates to Poor Prognosis and Promotes Proliferation, Stemness, Migration, and Oxidative Stress Resistance in Cholangiocarcinoma.

International journal of molecular sciences

Kaewlert W,Sakonsinsiri C,Lert-Itthiporn W,Ungarreevittaya P,Pairojkul C,Pinlaor S,Murata M,Thanan R

Thu Jan 26 00:00:00 EST 2023

Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes.

BMC medicine

Gautheron J,Lima L,Akinci B,Zammouri J,Auclair M,Ucar SK,Ozen S,Altay C,Bax BE,Nemazanyy I,Lenoir V,Prip-Buus C,Acquaviva-Bourdain C,Lascols O,Fève B,Vigouroux C,Noel E,Jéru I

Mon Mar 28 00:00:00 EDT 2022

MiR-195 promotes pancreatic β-cell dedifferentiation by targeting Mfn2 and impairing Pi3k/Akt signaling in type 2 diabetes.

Obesity (Silver Spring, Md.)

Xu Y,Tang Z,Dai H,Hou J,Li F,Tang Z,Zhang D

Tue Feb 01 00:00:00 EST 2022

Ginsenoside compound K inhibits obesity-induced insulin resistance by regulation of macrophage recruitment and polarization via activating PPARγ.

Food & function

Xu J,Dong J,Ding H,Wang B,Wang Y,Qiu Z,Yao F

Mon Mar 21 00:00:00 EDT 2022

Effect of 1-Deoxynojirimycin on insulin resistance in prediabetic mice based on next-generation sequencing and intestinal microbiota study.

Journal of ethnopharmacology

Ren X,Xing Y,He L,Xiu Z,Yang L,Han A,Jia Q,Dong Y

Tue May 10 00:00:00 EDT 2022

Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells.

Genetics research

Lin L,Wang X,Zhao W,Chen Y

Mon Nov 07 00:00:00 EST 2022

Insulin receptor substrate 1(IRS1) is related with lymph node metastases and prognosis in esophageal squamous cell carcinoma.

Gene

Lei Y,Jamal M,Zeng X,He H,Xiao D,Zhang C,Zhang X,Tan H,Xie S,Zhang Q

Mon Aug 15 00:00:00 EDT 2022

Insulin receptor substrate 1(IRS1) is related with lymph node metastases and prognosis in esophageal squamous cell carcinoma.

Gene

Lei Y,Jamal M,Zeng X,He H,Xiao D,Zhang C,Zhang X,Tan H,Xie S,Zhang Q

Mon Aug 15 00:00:00 EDT 2022

Fermented noni (Morinda citrifolia L.) fruit juice improved oxidative stress and insulin resistance under the synergistic effect of Nrf2/ARE pathway and gut flora in db/db mice and HepG2 cells.

Food & function

Zhang X,Zhang B,Li L,Li X,Zhang J,Chen G

Mon Aug 01 00:00:00 EDT 2022

Trimetazidine and exercise offer analogous improvements to the skeletal muscle insulin resistance of mice through Nrf2 signaling.

BMJ open diabetes research & care

Zhang W,Dun Y,You B,Qiu L,Ripley-Gonzalez JW,Cheng J,Fu S,Li C,Liu S

Tue Mar 01 00:00:00 EST 2022

Exercise activates autophagy and regulates endoplasmic reticulum stress in muscle of high-fat diet mice to alleviate insulin resistance.

Biochemical and biophysical research communications

Cheng F,Dun Y,Cheng J,Ripley-Gonzalez JW,Jiang W,You B,Liu S

Sat Apr 23 00:00:00 EDT 2022

Spatiotemporal regulation of insulin signaling by liquid-liquid phase separation.

Cell discovery

Zhou K,Chen Q,Chen J,Liang D,Feng W,Liu M,Wang Q,Wang R,Ouyang Q,Quan C,Chen S

Tue Jul 05 00:00:00 EDT 2022

Spatiotemporal regulation of insulin signaling by liquid-liquid phase separation.

Cell discovery

Zhou K,Chen Q,Chen J,Liang D,Feng W,Liu M,Wang Q,Wang R,Ouyang Q,Quan C,Chen S

Tue Jul 05 00:00:00 EDT 2022

Effect of crude polysaccharide from seaweed, Dictyopteris divaricata (CDDP) on gut microbiota restoration and anti-diabetic activity in streptozotocin (STZ)-induced T1DM mice.

Gut pathogens

Siddiqui NZ,Rehman AU,Yousuf W,Khan AI,Farooqui NA,Zang S,Xin Y,Wang L

Sat Sep 17 00:00:00 EDT 2022

Black rice bran intake reduces phosphorylated tau levels and enhances insulin signaling in the brain of aged normal mice.

Bioscience, biotechnology, and biochemistry

Hara T,Toyama H,Ohata Y,Ikeuchi T,Nakamura S,Joh T,Ohtsubo K

Thu Oct 20 00:00:00 EDT 2022

Ginsenoside Rg3 ameliorates myocardial glucose metabolism and insulin resistance via activating the AMPK signaling pathway.

Journal of ginseng research

Ni J,Liu Z,Jiang M,Li L,Deng J,Wang X,Su J,Zhu Y,He F,Mao J,Gao X,Fan G

Tue Mar 01 00:00:00 EST 2022

Jatrorrhizine from Rhizoma Coptidis exerts an anti-obesity effect in db/db mice.

Journal of ethnopharmacology

He H,Deng J,Yang M,An L,Ye X,Li X

Tue Nov 15 00:00:00 EST 2022

RNA binding motif protein 3 (RBM3) promotes protein kinase B (AKT) activation to enhance glucose metabolism and reduce apoptosis in skeletal muscle of mice under acute cold exposure.

Cell stress & chaperones

Liu Y,Shi H,Hu Y,Yao R,Liu P,Yang Y,Li S

Tue Nov 01 00:00:00 EDT 2022

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Environmental health perspectives

Raber J,Perez R,Torres ERS,Krenik D,Boutros S,Patel E,Chlebowski AC,Torres ER,Perveen Z,Penn A,Paulsen DB,Bartlett MG,Jia E,Holden S,Hall R,Morré J,Wong C,Ho E,Choi J,Stevens JF,Noël A,Bobe G,Kisby G

Sat May 01 00:00:00 EDT 2021

Exercise improves lipid droplet metabolism disorder through activation of AMPK-mediated lipophagy in NAFLD.

Life sciences

Li H,Dun Y,Zhang W,You B,Liu Y,Fu S,Qiu L,Cheng J,Ripley-Gonzalez JW,Liu S

Sat May 15 00:00:00 EDT 2021

BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

Autophagy

da Silva Rosa SC,Martens MD,Field JT,Nguyen L,Kereliuk SM,Hai Y,Chapman D,Diehl-Jones W,Aliani M,West AR,Thliveris J,Ghavami S,Rampitsch C,Dolinsky VW,Gordon JW

Wed Sep 01 00:00:00 EDT 2021

Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats.

Cells

Cheng YC,Chiu YM,Dai ZK,Wu BN

Fri Oct 08 00:00:00 EDT 2021

EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence.

eLife

Gautheron J,Morisseau C,Chung WK,Zammouri J,Auclair M,Baujat G,Capel E,Moulin C,Wang Y,Yang J,Hammock BD,Cerame B,Phan F,Fève B,Vigouroux C,Andreelli F,Jeru I

Tue Aug 03 00:00:00 EDT 2021

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.

Molecular cell

Senoo H,Murata D,Wai M,Arai K,Iwata W,Sesaki H,Iijima M

Thu Nov 18 00:00:00 EST 2021

Ginsenoside Rg5 Improves Insulin Resistance and Mitochondrial Biogenesis of Liver via Regulation of the Sirt1/PGC-1α Signaling Pathway in db/db Mice.

Journal of agricultural and food chemistry

Zhu Y,Yang H,Deng J,Fan D

Wed Aug 04 00:00:00 EDT 2021

A maternal high-fat/low-fiber diet impairs glucose tolerance and induces the formation of glycolytic muscle fibers in neonatal offspring.

European journal of nutrition

Hu C,Yang Y,Chen M,Hao X,Wang S,Yang L,Yin Y,Tan C

Sun Aug 01 00:00:00 EDT 2021

DPHC From Alpinia officinarum Ameliorates Oxidative Stress and Insulin Resistance via Activation of Nrf2/ARE Pathway in db/db Mice and High Glucose-Treated HepG2 Cells.

Frontiers in pharmacology

Zhang X,Zhang Y,Zhou M,Xie Y,Dong X,Bai F,Zhang J

Fri Feb 04 00:00:00 EST 2022

Nano chitosan-zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets.

The British journal of nutrition

Zhang M,Hou G,Hu P,Feng D,Wang J,Zhu W

Tue Sep 28 00:00:00 EDT 2021

Pentadecanoic acid promotes basal and insulin-stimulated glucose uptake in C2C12 myotubes.

Food & nutrition research

Fu WC,Li HY,Li TT,Yang K,Chen JX,Wang SJ,Liu CH,Zhang W

Wed Apr 20 00:00:00 EDT 2022

Programmed cell death 5 improves skeletal muscle insulin resistance by inhibiting IRS-1 ubiquitination through stabilization of MDM2.

Life sciences

Li B,Ye J,Liu R,Weng L,Cao Y,Jia S,Xu C,Liu Y,Yan S,Zheng M

Mon Nov 15 00:00:00 EST 2021

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Clinical and translational medicine

Liu AD,Zhou J,Bi XY,Hou GQ,Li SS,Chen Q,Xu H,Cao X

Mon Mar 01 00:00:00 EST 2021

The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2.5 Instillation-Induced Insulin Resistance in Wistar Rats.

Disease markers

Zhang Z,Hu S,Fan P,Li L,Feng S,Xiao H,Zhu L

Wed Feb 23 00:00:00 EST 2022

miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1.

BMC cancer

Meng Y,Hu X,Li S,Zeng X,Qiu L,Wei M,Wang Z,Han J

Tue Oct 27 00:00:00 EDT 2020

CEBPG promotes esophageal squamous cell carcinoma progression by enhancing PI3K-AKT signaling.

American journal of cancer research

Huang Y,Lin L,Shen Z,Li Y,Cao H,Peng L,Qiu Y,Cheng X,Meng M,Lu D,Yin D

Thu Nov 12 00:00:00 EST 2020

Eurocristatine, a plant alkaloid from Eurotium cristatum, alleviates insulin resistance in db/db diabetic mice via activation of PI3K/AKT signaling pathway.

European journal of pharmacology

Zhang H,Hui J,Yang J,Deng J,Fan D

Sun Nov 15 00:00:00 EST 2020

Prenatal High-Salt Diet-Induced Metabolic Disorders via Decreasing Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1α in Adult Male Rat Offspring.

Molecular nutrition & food research

Liu Y,Yang C,Feng X,Qi L,Guo J,Zhu D,Thai PN,Zhang Y,Zhang P,Sun M,Lv J,Zhang L,Xu Z,Lu X

Wed Jul 01 00:00:00 EDT 2020

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

Fish physiology and biochemistry

Liang H,Mokrani A,Chisomo-Kasiya H,Ji K,Ge X,Ren M,Liu B,Xi B,Sun A

Mon Apr 01 00:00:00 EDT 2019

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

Fish physiology and biochemistry

Liang H,Mokrani A,Chisomo-Kasiya H,Ji K,Ge X,Ren M,Liu B,Xi B,Sun A

Mon Apr 01 00:00:00 EDT 2019

Suppression of miR-143 contributes to overexpression of IL-6, HIF-1α and NF-κB p65 in Cr(VI)-induced human exposure and tumor growth.

Toxicology and applied pharmacology

Wang L,Qiu JG,He J,Liu WJ,Ge X,Zhou FM,Huang YX,Jiang BH,Liu LZ

Sun Sep 01 00:00:00 EDT 2019

Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.

Toxicology

Wang F,Chang C,Li R,Zhang Z,Jiang H,Zeng N,Li D,Chen L,Xiao Y,Chen W,Wang Q

Thu Aug 01 00:00:00 EDT 2019

Hydroxychloroquine Improves Obesity-Associated Insulin Resistance and Hepatic Steatosis by Regulating Lipid Metabolism.

Frontiers in pharmacology

Qiao X,Zhou ZC,Niu R,Su YT,Sun Y,Liu HL,Teng JL,Ye JN,Shi H,Yang CD,Cheng XB

Tue Sep 29 00:00:00 EDT 2020

Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yang S,Chen Y,Duan Y,Ma C,Liu L,Li Q,Yang J,Li X,Zhao B,Wang Y,Qian K,Liu M,Zhu Y,Yang X,Han J

Tue Oct 01 00:00:00 EDT 2019

miR-146b Inhibits Glucose Consumption by Targeting IRS1 Gene in Porcine Primary Adipocytes.

International journal of molecular sciences

Zhu YL,Chen T,Xiong JL,Wu D,Xi QY,Luo JY,Sun JJ,Zhang YL

Fri Mar 09 00:00:00 EST 2018

miR-146b Inhibits Glucose Consumption by Targeting IRS1 Gene in Porcine Primary Adipocytes.

International journal of molecular sciences

Zhu YL,Chen T,Xiong JL,Wu D,Xi QY,Luo JY,Sun JJ,Zhang YL

Fri Mar 09 00:00:00 EST 2018

DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.

International journal of biological sciences

Xu M,Wu H,Li M,Wen Y,Yu C,Xia L,Xia Q,Kong X

Mon Sep 09 00:00:00 EDT 2019

DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.

International journal of biological sciences

Xu M,Wu H,Li M,Wen Y,Yu C,Xia L,Xia Q,Kong X

Mon Sep 09 00:00:00 EDT 2019

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Scientific reports