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Invitrogen
IRS1 Recombinant Superclonal Antibody (21HCLC)
Advanced Verification
IP-mass spectrometry
Product Details
710009
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
21HCLC
Immunogen
Recombinant protein corresponding to amino acids 694-875.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532522
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: HIRS-1; IRS1
UniProt ID: (Human) P35568
Entrez Gene ID: (Human) 3667
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
phosphatidylinositol-3-phosphate biosynthetic process
glucose homeostasis
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of glucose import in response to insulin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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