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Invitrogen

ISL1 Polyclonal Antibody

Name: ISL1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA527789
Price: 540.0 USD
Availability: InStock

Advanced Verification

1 Published Figure

3 References

View all (44) ISL1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

ISL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

ISL1 Antibody (PA5-27789) in ICC/IF

Immunofluorescence analysis of ISL1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ISL1 Polyclonal Antibody (Product # PA5-27789) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A2... View More

ISL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISL1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

ISL1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ISL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of Islet-1 was observed in SH-SY5Y as compared to THP1 cells using Anti-Islet-1 Polyclonal Antibody (Product # PA5-27789) in western blot. {RE}

Product Details

PA5-27789

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545265

Product Specific Information

Recommended positive controls: K562 whole cell extract, K562 nuclear extract, jurkat whole cell extract, jurkat nuclear extract, PC-12.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Japanese Medaka (97%), Xenopus laevis (94%), Chicken (99%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR/Cas9 Directed Reprogramming of iPSC for Accelerated Motor Neuron Differentiation Leads to Dysregulation of Neuronal Fate Patterning and Function.

International journal of molecular sciences

Davis-Anderson K,Micheva-Viteva S,Solomon E,Hovde B,Cirigliano E,Harris J,Twary S,Iyer R

Published figure using ISL1 polyclonal antibody (Product # PA5-27789) in Immunohistochemistry

Fri Nov 10 00:00:00 UTC 2023

CRISPR/Cas9 Directed Reprogramming of iPSC for Accelerated Motor Neuron Differentiation Leads to Dysregulation of Neuronal Fate Patterning and Function.

International journal of molecular sciences

Davis-Anderson K,Micheva-Viteva S,Solomon E,Hovde B,Cirigliano E,Harris J,Twary S,Iyer R

Published figure using ISL1 polyclonal antibody (Product # PA5-27789) in Immunohistochemistry

Fri Nov 10 00:00:00 UTC 2023

Temporal Expression of Hox Genes and Phox2b in the Rat Nucleus Ambiguus During Development: Implications on Laryngeal Innervation.

The Laryngoscope

Honzel E,Hernandez-Morato I,Joshi A,Pennington-Fitzgerald W,Moayedi Y,Pitman MJ

PA5-27789 was used in Immunohistochemistry to suggest the involvement of Hoxb1, Hoxb2 and their target Phox2b in cueing laryngeal motoneuron identity and target identification, while higher expression of Phox2b at earlier stages implies a role in laryngeal motoneuron formation.

Fri Dec 01 00:00:00 UTC 2023

Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Chase R,de la Peña JB,Smith PR,Lawson J,Lou TF,Stanowick AD,Black BJ,Campbell ZT

PA5-27789 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to reveal hypusine as a potential target for inflammation associated pain in males.

Fri Jul 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: Insulin gene enhancer protein ISL-1; insulin related protein; isl-1 homeobox; ISL1 transcription factor LIM/homeodomain (islet-1); ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain 1; Islet 1; Islet-1; islet1; Lim-homeodomain protein Islet1

Gene Aliases: Isl-1; isl-1=homeobox; ISL1; islet-1; ISLET1; si:ch211-219f7.1

UniProt ID: (Human) P61371, (Mouse) P61372, (Rat) P61374, (Zebrafish) P53405

Entrez Gene ID: (Human) 3670, (Mouse) 16392, (Rat) 64444, (Zebrafish) 30147

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding chromatin binding protein binding zinc ion binding ligand-dependent nuclear receptor binding estrogen receptor binding bHLH transcription factor binding DNA binding sequence-specific DNA binding metal ion binding RNA polymerase II transcription factor activity, sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ISL1 Polyclonal Antibody

ISL1 Antibody (PA5-27789) in ICC/IF

Product Details

PA5-27789

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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ISL1 Polyclonal Antibody

ISL1 Antibody (PA5-27789) in ICC/IF

Product Details

PA5-27789

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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