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ITGB8 Antibody (PA5-55692) in ICC/IF
Immunofluorescence analysis of ITGB8 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ITGB8 Polyclonal Antibody (Product # PA5-55692) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
PA5-55692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ITGB8. Recombinant protein control fragment (Product #RP-95271).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2642816
Product Specific Information
Immunogen sequence: AQHCVNSKGQ VCSGRGTCVC GRCECTDPRS IGRFCEHCPT CYTACKENWN CMQCLHPHNL SQAILDQCKT SCALMEQQHY
Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 85%.
Target Information
ITGB8 (Integrin beta 8) is a member of the integrin beta chain family and is a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: integrin beta 8; Integrin beta-8; Integrin beta8; integrin, beta 8
Gene Aliases: ITGB8
UniProt ID: (Human) P26012
Entrez Gene ID: (Human) 3696
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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