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Invitrogen
ITIH1 Polyclonal Antibody
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Application
FIGURE: 1 / 5
ITIH1 Antibody (PA1-1755) in ICC/IF
Immunofluorescence analysis of ITIH1 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ITIH1 Rabbit Polyclonal Antibody (Product # PA1-1755) at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Product Details
PA1-1755
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues Q V Q E S L P E L S N.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2129516
Product Specific Information
PA1-1755 detects HC1 from human and mouse samples.
PA1-1755 has been successfully used in immunofluorescence and immunohistochemical staining of paraffin embedded tissue sections.
The PA1-1755 immunogen is a synthetic peptide corresponding to residues Q V Q E S L P E L S N. This immunizing peptide (PEP-303) is available for neutralization and control experiments.
PA1-1755 can be used with blocking peptide PEP-301.
Target Information
The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CYCSP39; HCP39; inter-alpha (globulin) inhibitor, H1 polypeptide; inter-alpha-inhibitor heavy chain 1; Inter-alpha-trypsin inhibitor complex component III; Inter-alpha-trypsin inhibitor heavy chain H1; ITI heavy chain H1; MGC126415; Serum-derived hyaluronan-associated protein; SHAP
Gene Aliases: H1P; IATIH; IGHEP1; Intin1; ITI-HC1; ITIH; Itih-1; ITIH1; SHAP
UniProt ID: (Human) P19827, (Mouse) Q61702
Entrez Gene ID: (Human) 3697, (Mouse) 16424
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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