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Invitrogen
Influenza A M2 Polyclonal Antibody
Application
FIGURE: 1 / 6
Influenza A M2 Antibody (PA5-32233) in ICC/IF
Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A Virus M2 antibody (Product # PA5-32233) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.
Product Details
PA5-32233
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Influenza A virus M2 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.61 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549706
Product Specific Information
Recommended positive controls: Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates, H1N1-infected H1299.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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