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            • Primary Antibodies ›
            • LPAM-1 Antibodies

            Invitrogen

            Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), APC, eBioscience™

            1 Published Figure
            11 References
            View all (14) LPAM-1 antibodies
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            Catalog # 17-5887-82
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            Price (USD)
            357.00
            100 µg
             
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            Cite Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), APC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (1)
            Application
            Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            Integrin alpha 4 beta 7 (LPAM-1) Antibody (17-5887-82) in Flow

            Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse Integrin alpha 4 beta 7 (LPAM-1) APC (right). Total viable cells were used for analysis.
            View Product

            Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)
            Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)

            Product Details

            17-5887-82

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.25 µg/test
            View 11 publications 11 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            DATK32 (DATK-32)

            Conjugate

            APC
            • Unconjugated
            • Biotin 
            • Functional Grade
            • PE 
            • PerCP-eFluor 710
            • Super Bright 436
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_1210577

            Product Specific Information

            Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

            Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; Integrin beta-7; Integrin beta-P; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA4 subunit alpha

            View more View less

            Gene Aliases: CD49D; Itga4; Itga4B; Itgb7; Ly69

            View more View less

            UniProt ID: (Mouse) Q00651, (Mouse) P26011

            View more View less

            Entrez Gene ID: (Mouse) 16401, (Mouse) 16421

            View more View less

            Function(s)

            fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding receptor activity integrin

            Process(es)

            blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration leukocyte migration
            It has to be done as per old AB suggested Products section.
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