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Rockland

Integrin beta 3 Polyclonal Antibody

View all (104) CD61 (Integrin beta 3) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

Integrin beta 3 Antibody in Western Blot (WB)

FIGURE: 1 / 1

Integrin beta 3 Antibody (600-401-GU0) in WB

Western Blot of Rabbit anti-Integrin beta 3 antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: HUVEC whole cell lysate. Lane 3: U87 whole cell lysate (W09-001-GX2). Lane 4: MDA-MB-435 whole cell lysate p/n (W09-001-A39). Load: 35 µg per lane. Primary antibody: Integrin beta 3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody p/n (611-103-122) at 1:10,000 for 45 min at RT. Block: BlockOut buffer - p/n (MB-073) overnight at 4°C. Predicted/Observed size: ~87kDa/~80, 100 and... View More

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Product Details

600-401-GU0

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

1:100-1:200

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

5.0 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Integrin beta 3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human Integrin beta 3 conjugated to Keyhole Limpet Hemocyanin (KLH).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Integrin beta 3. Blast analysis of the sequence of the immunogen shows 100% identity with human and mouse.

Target Information

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antigen CD61; CD61; GPIIIa; integrin beta 3; Integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa

Gene Aliases: BDPLT16; BDPLT2; CD61; GP3A; GPIIIa; GT; ITGB3

UniProt ID: (Human) P05106

Entrez Gene ID: (Human) 3690

Function(s)

virus receptor activity fibronectin binding protease binding protein disulfide isomerase activity platelet-derived growth factor receptor binding protein binding coreceptor activity fibroblast growth factor binding enzyme binding C-X3-C chemokine binding insulin-like growth factor I binding neuregulin binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding integrin

Process(es)

positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation platelet degranulation cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway blood coagulation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage smooth muscle cell migration cell growth cell migration platelet activation extracellular matrix organization positive regulation of vascular endothelial growth factor receptor signaling pathway cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading tube development apolipoprotein A-I-mediated signaling pathway wound healing regulation of bone resorption negative regulation of low-density lipoprotein particle receptor biosynthetic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway mesodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process leukocyte migration negative chemotaxis angiogenesis involved in wound healing platelet aggregation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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