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Invitrogen
J Chain Monoclonal Antibody (SP105)
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Application
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J Chain Antibody (MA5-16419) in IHC (P)
Immunohistochemical (paraffin) analysis of J-chain using anti-J-chain Monoclonal Antibody (Product # MA5-16419) in Tonsil Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:100.
Product Details
MA5-16419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP105
Immunogen
Synthetic peptide near the internal region of human J-chain.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. not determined
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537938
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
Target Information
J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgJ chain; Immunoglobulin J chain; immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides; J chain; Joining chain of multimeric IgA and IgM
Gene Aliases: IGCJ; IGJ; JCH; JCHAIN
UniProt ID: (Human) P01591
Entrez Gene ID: (Human) 3512
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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