Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
KEAP1 Antibody (PA5-142760) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of KEAP1 in HeLa cells using KEAP1 Polyclonal Antibody (Product # PA5-142760). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142760
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-EVTPSQHGNRTFS.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933406
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Cytosolic inhibitor of Nrf2; INrf2; Kelch-like ECH-associated protein 1; kelch-like family member 19; Kelch-like protein 19
Gene Aliases: INRF2; KEAP1; KIAA0132; KLHL19
UniProt ID: (Human) Q14145
Entrez Gene ID: (Human) 9817
in utero embryonic development
selenium compound metabolic process
malate metabolic process
transcription, DNA-templated
flavonoid metabolic process
response to metal ion
proteasomal ubiquitin-independent protein catabolic process
negative regulation of gene expression
protein ubiquitination
alkanesulfonate metabolic process
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to immobilization stress
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
cytoplasmic sequestering of transcription factor
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of epidermal cell differentiation
protein oligomerization
cellular response to interleukin-4
cellular response to organic cyclic compound
response to thyroid hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support