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Application
FIGURE: 1 / 1
KIAA0101 Antibody (PA5-87838) in WB
Western blot analysis of KIAA0101 in extracts of various cell lines. Samples were incubated with KIAA0101 Polyclonal antibody (Product # PA5-87838) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-87838
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 39-111 of human KIAA0101 (NP_0555511)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.34 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804445
Product Specific Information
Immunogen sequence: SSRKAENKYA GGNPVCVRPT PKWQKGIGEF FRLSPKDSEK ENQIPEEAGS SGLGKAKRKA CPLQPDHTND EKE
Positive Samples: HT-29, K-562; Cellular Location: Cytoplasm, Nucleus, perinuclear region
Target Information
PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HCV NS5A-transactivated protein 9; Hepatitis C virus NS5A-transactivated protein 9; OEATC-1; Overexpressed in anaplastic thyroid carcinoma 1; PAF15; PCNA-associated factor; PCNA-associated factor of 15 kDa; PCNA-clamp-associated factor
Gene Aliases: KIAA0101; L5; NS5ATP9; OEATC; OEATC-1; OEATC1; p15(PAF); p15/PAF; p15PAF; PAF; PAF15; PCLAF
UniProt ID: (Human) Q15004
Entrez Gene ID: (Human) 9768
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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