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Invitrogen

KID Monoclonal Antibody (1E3)

5 Published Figures

View all (10) KID antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (5)
Published Figures (5)

Application

KID Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

KID Antibody (MA5-15912) in IHC (P)

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using kid monoclonal antibody (Product # MA5-15912) followed with DAB staining.

KID Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KID Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KID Antibody in Western Blot (WB)

KID Antibody in Western Blot (WB)

KID Antibody in Flow Cytometry (Flow)

KID Antibody in Western Blot, Immunohistochemistry (WB, IHC)

KID Antibody in Immunohistochemistry (IHC)

KID Antibody in Immunohistochemistry (IHC)

KID Antibody in Western Blot (WB)

KID Antibody in ChIP Assay (ChIP)

Product Image Gallery

KID Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using kid monoclonal antibody (Product # MA5-15912) followed with DAB staining.

KID Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded colon cancer tissues using kid monoclonal antibody (Product # MA5-15912) followed with DAB staining.

KID Antibody in Western Blot (WB) — Western blot analysis of kid using kid monoclonal antibody (Product # MA5-15912) in HEK293 (1) and kid (AA: 225-419) human IgG Fc transfected HEK293 (2) cell lysate.

KID Antibody in Western Blot (WB) — Western blot analysis of kid using a kid monoclonal antibody (Product # MA5-15912) against a human kid (AA: 225-419) recombinant protein.

KID Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of NIH/3T3 cells using kid monoclonal antibody (Product # MA5-15912) (blue) and negative control (red).

KID Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5 KIF22 promotes bladder tumor growth in mice. (A) Representative images of tumors in nude mice formed by T24 cells transfected with shControl and shKIF22 lentivirus (n=8 in each group). (B) Tumor volume was measured and growth curves are presented. (C) Western blot analysis revealed the expression of KIF22 in the control and KIF22-silenced tumor tissues. (D) Immunohistochemistry revealed the expression of KIF22 in the tumor tissues from mice injected with control and KIF22-silenced cells. The results are presented as the mean +- SEM; * P<0.05 and ** P<0.01, compared with the shControl. KIF22, kinesin family member 22.

KID Antibody in Immunohistochemistry (IHC) — Figure 1 KIF22 is associated with the prognosis of patients with bladder cancer. (A) Left panel, representative images of KIF22 expression detected by immunohistochemistry in bladder cancer tissues (x100 and x200 magnification). Right panel, immunohistochemical staining of KIF22 protein in normal adjacent tissues (x100 and x200 magnification). (B) Bioinformatics data revealed that KIF22 was highly expressed in human bladder cancer tissues. (C) Kaplan-Meier analysis of the overall survival and disease-free survival rate between the KIF22 low and high expression groups. KIF22, kinesin family member 22.

KID Antibody in Immunohistochemistry (IHC) — Figure 7 CDCA3 expression is positively associated with KIF22 expression in surgical bladder cancer tissues of patients. (A) Representative images of immunohistochemical staining of CDCA3 and KIF22 in bladder cancer tissues. (B) Pearson's correlation analysis of the significance between the expression of KIF22 and CDCA3 in human bladder cancer tissues. KIF22, kinesin family member 22; cell division cycle-associated protein 3.

KID Antibody in Western Blot (WB) — Figure 2 KIF22 expression is efficiently blocked by KIF22 shRNA in both T24 and 5637 cells. (A) Results of reverse transcription-quantitative PCR revealed that KIF22 expression was efficiently knocked down in T24 and 5637 cells caused by transfection with KIF22 shRNA plasmids. (B) Western blot analysis illustrating that KIF22 expression was markedly decreased in KIF22-shRNA transfected T24 and 5637 cells. The experiments were repeated three times. The results are presented as the mean +- SEM; * P<0.05 and ** P<0.01, compared with the shControl. KIF22, kinesin family member 22.

KID Antibody in ChIP Assay (ChIP) — Figure 6 KIF22 transcriptionally activates the expression of CDCA3. (A) CDCA3 mRNA expression in the T24 (left panel) or 5637 (right panel) cells transfected with the pcDNA3.1-vector, pcDNA3.1-KIF22, control or KIF22 shRNA plasmids examined using reverse transcription-quantitative PCR. (B) CDCA3 expression in T24 cells transfected with pcDNA3.1-vector, pcDNA3.1-KIF22, control or KIF22 shRNA plasmids examined using western blot analysis. (C) CCK-8 assays exhibited the difference in the proliferation of the T24 (left panel) and 5637 (right panel) cells transfected with the indicated shRNAs and/or plasmids. (D) The effects of KIF22 on proliferation in T24 cells transfected the indicated shRNAs or plasmids were detected using colony formation assays, and the colony numbers were quantified. (E) Luciferase activity of pGL3-Basic, pGL3-CDCA3 in T24 cells co-transfected with pcDNA3.1-KIF22 or pcDNA3.1-vector plasmids examined using by dual-luciferase reporter assays. (F) PCR amplification of the anti-KIF22 antibody-enriched CDCA3 promoter fragment in T24 cells using ChIP assay. (G) Western blot analysis revealed the difference in Ki67 expression in the T24 (left panel) and 5637 (right panel) cells transfected with the indicated shRNAs and/or plasmids. The in vitro independent experiments were repeated three times. The results are presented as the mean +- SEM, * P<0.05. ** P<0.01 and *** P<0.001; ns, not significant; KIF22, kinesin family member 22; cell division cycle-associated prot

Product Details

MA5-15912

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E3

Immunogen

Purified recombinant fragment of human KID expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154373

Product Specific Information

MA5-15912 targets KID in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15912 immunogen is purified recombinant fragment of human KID expressed in E. Coli. .

MA5-15912 detects KID which has a predicted molecular weight of approximately 73kDa.

Target Information

The protein encoded by this gene is a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

KIF22 promotes bladder cancer progression by activating the expression of CDCA3.

International journal of molecular medicine

Li K,Li S,Tang S,Zhang M,Ma Z,Wang Q,Chen F

Published figure using KID monoclonal antibody (Product # MA5-15912) in Immunohistochemistry

Wed Dec 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: kinesin family member 22A; kinesin-like 4; Kinesin-like DNA-binding protein; kinesin-like DNA-binding protein pseudogene; Kinesin-like protein 4; Kinesin-like protein KIF22; origin of plasmid DNA replication-binding protein; oriP binding protein

Gene Aliases: A-328A3.2; AU021460; C81217; KID; KIF22; Kif22a; KNSL4; OBP; OBP-1; OBP-2; SEMDJL2

UniProt ID: (Human) Q14807, (Mouse) Q3V300

Entrez Gene ID: (Human) 3835, (Mouse) 110033

Function(s)

DNA binding microtubule motor activity protein binding ATP binding microtubule binding ATPase activity nucleotide binding microtubule binding motor protein

Process(es)

DNA repair retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement sister chromatid cohesion mitotic nuclear division mitotic metaphase plate congression antigen processing and presentation of exogenous peptide antigen via MHC class II metaphase plate congression

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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