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Invitrogen

LC3B Polyclonal Antibody

Name: LC3B Polyclonal Antibody
Brand: Invitrogen
SKU: PA532254
Price: 586.0 USD
Availability: InStock

Advanced Verification

2 Published Figures

7 References

View all (56) LC3B antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

LC3B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

LC3B Antibody (PA5-32254) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of LC3B in HeLa cells mock (left) and in HeLa cells treated with 50 µM Chloroquine for 24 hr (right) fixed with 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:2,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.

LC3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LC3B Antibody in Immunoprecipitation (IP)

Product Image Gallery

LC3B Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-LC3B Polyclonal Antibody (Product # PA5-32254), shows induced expression in HeLa and HCT 116 cells upon Chloroquine treatment. {TM}

Product Details

PA5-32254

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:200

Immunoprecipitation (IP)

1:100-1:500

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Human, Insect, Mouse, Pig, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2549727

Product Specific Information

Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse brain, Rat brain, HepG2 (untreated), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 16hr), HepG2 (3 µM Thapsigargin treatment for 24hr), Huh7 (un-infected), Huh7 (HCV-infected), HeLa, HeLa (50 µM Chloroquine treatment for 24 hr).

Predicted reactivity: Zebrafish (100%), Japanese Medaka (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice.

Frontiers in oncology

Testa MTJ,Cella PS,Marinello PC,Frajacomo FTT,Padilha CS,Perandini PC,Moura FA,Duarte JA,Cecchini R,Guarnier FA,Deminice R

PA5-32254 was used in Western Blotting to investigate the effects of resistance exercise training on inflammatory cytokines and oxidative-mediated STAT3 activation and muscle loss prevention in tumor-bearing mice.

Tue Jul 19 00:00:00 UTC 2022

ACSS3 in brown fat drives propionate catabolism and its deficiency leads to autophagy and systemic metabolic dysfunction.

Clinical and translational medicine

Jia Z,Chen X,Chen J,Zhang L,Oprescu SN,Luo N,Xiong Y,Yue F,Kuang S

PA5-32254 was used in Immunocytochemistry to establish ACSS3 as the key enzyme for propionate metabolism and demonstrate that accumulation of propionate promotes obesity and Type 2 diabetes through triggering adipocyte autophagy.

Tue Feb 01 00:00:00 UTC 2022

ACSS3 in brown fat drives propionate catabolism and its deficiency leads to autophagy and systemic metabolic dysfunction.

Clinical and translational medicine

Jia Z,Chen X,Chen J,Zhang L,Oprescu SN,Luo N,Xiong Y,Yue F,Kuang S

Tue Feb 01 00:00:00 UTC 2022

ACSS3 in brown fat drives propionate catabolism and its deficiency leads to autophagy and systemic metabolic dysfunction.

Clinical and translational medicine

Jia Z,Chen X,Chen J,Zhang L,Oprescu SN,Luo N,Xiong Y,Yue F,Kuang S

Tue Feb 01 00:00:00 UTC 2022

Ghrelin ameliorates chronic obstructive pulmonary disease-associated infllammation and autophagy.

Biotechnology and applied biochemistry

Song B,Yan X,Li R,Zhang H

PA5-32254 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that ghrelin exhibits beneficial effects on patients with COPD.

Thu Apr 01 00:00:00 UTC 2021

Low Radiation Environment Switches the Overgrowth-Induced Cell Apoptosis Toward Autophagy.

Frontiers in public health

Fischietti M,Fratini E,Verzella D,Vecchiotti D,Capece D,Di Francesco B,Esposito G,Balata M,Ioannuci L,Sykes P,Satta L,Zazzeroni F,Tessitore A,Tabocchini MA,Alesse E

PA5-32254 was used in Western Blot to investigate the core mechanisms putatively responsible for different cellular behaviors attributable to a low radiation environment.

Fri May 14 00:00:00 UTC 2021

Low Radiation Environment Switches the Overgrowth-Induced Cell Apoptosis Toward Autophagy.

Frontiers in public health

Fischietti M,Fratini E,Verzella D,Vecchiotti D,Capece D,Di Francesco B,Esposito G,Balata M,Ioannuci L,Sykes P,Satta L,Zazzeroni F,Tessitore A,Tabocchini MA,Alesse E

PA5-32254 was used in Western Blot to investigate the core mechanisms putatively responsible for different cellular behaviors attributable to a low radiation environment.

Fri May 14 00:00:00 UTC 2021

Stimulation of triple negative breast cancer cell migration and metastases formation is prevented by chloroquine in a pre-irradiated mouse model.

BMC cancer

Bouchard G,Therriault H,Geha S,Bérubé-Lauzière Y,Bujold R,Saucier C,Paquette B

PA5-32254 was used in western blot to utilize chloroquine in a pre-irradiated mouse model to prevent cancer cell migration and metastases formation in a simulation of triple negative breast cancer

Fri Jun 10 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: ATG8E; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3 beta; LC3A; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1

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Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; Map1lc3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; zbs559

UniProt ID: (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q62625, (Mouse) Q9CQV6

Entrez Gene ID: (Human) 81631, (Pig) 100462754, (Human) 643246, (Rat) 64862, (Mouse) 67443

Function(s)

protein binding microtubule binding ubiquitin protein ligase binding tubulin binding protein domain specific binding GABA receptor binding non-motor microtubule binding protein

Process(es)

autophagosome assembly cellular response to nitrogen starvation macroautophagy regulation of mitophagy mitophagy autophagy positive regulation of protein binding nucleophagy membrane fusion positive regulation of mucus secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LC3B Polyclonal Antibody

LC3B Antibody (PA5-32254) in ICC/IF

Product Details

PA5-32254

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions