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LIMK2 Polyclonal Antibody

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Antibody Testing Data (1)

FIGURE: 1 / 1

LIMK2 Antibody (12350-1-AP) in WB

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12350-1-AP ( LIMK2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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Product Details

12350-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LIMK2 Fusion Protein Ag3011 (379-686 aa encoded by BC013051)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KLNLLTEYI EGGTLKDFLR SMDPFPWQQK VRFAKGIASG MAYLHSMCII HRDLNSHNCL IKLDKTVVVA DFGLSRLIVE ERKRAPMEKA TTKKRTLRKN DRKKRYTVVG NPYWMAPEML NGKSYDETVD IFSFGIVLCE IIGQVYADPD CLPRTLDFGL NVKLFWEKFV PTDCPPAFFP LAAICCRLEP ESRAPPGAAG EGPGCADDEG PVRRQGKVTI KYDPKELRKH LNLEEWILEQ LTRLYDCQEE EISELEIDVD ELLDMESDDA WASRVKELLV DCYKPTEAFI SGLLDKIRAM QKLSTPQKK (379-686 aa encoded by BC013051)

Target Information

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The identification of a two-gene prognostic model based on cisplatin resistance-related ceRNA network in small cell lung cancer.

BMC medical genomics

Zhang Y,Zhu Q,Qi J,Fu M,Xu A,Wang W,Wang H,Nie J,Hong B

Mon May 15 00:00:00 EDT 2023

RhoA/ROCK2 signalling is enhanced by PDGF-AA in fibro-adipogenic progenitor cells: implications for Duchenne muscular dystrophy.

Journal of cachexia, sarcopenia and muscle

Fernández-Simón E,Suárez-Calvet X,Carrasco-Rozas A,Piñol-Jurado P,López-Fernández S,Pons G,Bech Serra JJ,de la Torre C,de Luna N,Gallardo E,Díaz-Manera J

Fri Apr 01 00:00:00 EDT 2022

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Advanced healthcare materials

Deng M,Tan J,Hu C,Hou T,Peng W,Liu J,Yu B,Dai Q,Zhou J,Yang Y,Dong R,Ruan C,Dong S,Xu J

Wed Jul 01 00:00:00 EDT 2020

NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.

Theranostics

Xiong M,Chen L,Zhou L,Ding Y,Kazobinka G,Chen Z,Hou T

Wed Sep 16 00:00:00 EDT 2020

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Cell death & disease

Zhang Y,Li A,Shi J,Fang Y,Gu C,Cai J,Lin C,Zhao L,Liu S

Tue Jul 03 00:00:00 EDT 2018

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Cell death & disease

Zhang Y,Li A,Shi J,Fang Y,Gu C,Cai J,Lin C,Zhao L,Liu S

Tue Jul 03 00:00:00 EDT 2018

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Cell death & disease

Zhang Y,Li A,Shi J,Fang Y,Gu C,Cai J,Lin C,Zhao L,Liu S

Tue Jul 03 00:00:00 EDT 2018

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Cell death & disease

Zhang Y,Li A,Shi J,Fang Y,Gu C,Cai J,Lin C,Zhao L,Liu S

Tue Jul 03 00:00:00 EDT 2018

ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement.

PloS one

Choi HJ,Sanders TA,Tormos KV,Ameri K,Tsai JD,Park AM,Gonzalez J,Rajah AM,Liu X,Quinonez DM,Rinaudo PF,Maltepe E

Fri Aug 23 00:00:00 EDT 2013

ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement.

PloS one

Choi HJ,Sanders TA,Tormos KV,Ameri K,Tsai JD,Park AM,Gonzalez J,Rajah AM,Liu X,Quinonez DM,Rinaudo PF,Maltepe E

Fri Aug 23 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIM kinase 2; LIM motif-containing protein kinase 2; LIMK; LIMK 2; LIMK-2; LIMK1; LINhp-2; OTTHUMP00000199483

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Gene Aliases: Limk-2; LIMK2; Limk2b; Link2

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UniProt ID: (Human) P53671, (Mouse) O54785, (Rat) P53670

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Entrez Gene ID: (Human) 3985, (Mouse) 16886, (Rat) 29524

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Function(s)

protein serine/threonine kinase activity protein binding ATP binding zinc ion binding protein heterodimerization activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding

Process(es)

protein phosphorylation spermatogenesis phosphorylation spermatid development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LIMK2 Polyclonal Antibody

LIMK2 Antibody (12350-1-AP) in WB

Product Details

12350-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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