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Invitrogen

LRP1 Recombinant Rabbit Monoclonal Antibody (SA0290)

Advanced Verification

1 Published Figure

4 References

View all (55) LRP1 (CD91) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

LRP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

LRP1 Antibody (MA5-31959) in ICC/IF

Immunocytochemical analysis of LRP1 in MCF-7 cells using a LRP1 Monoclonal antibody (Product # MA5-31959) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

LRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LRP1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

LRP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

LRP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of LRP1 was observed among the breast cancer cell lines: MDA-MB 231, a cell model positive for LRP1 expression, in comparison to SK-BR-3, a negative cell model for LRP1 expression using Anti-LRP1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-31959) in western blot. Relative expression was also observed across mouse tissues tested, where LRP1 is highly expressed in liver as compared to heart. {RE}

Product Details

MA5-31959

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000-1:5,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Immunoprecipitation (IP)

1-2 µg/sample

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SA0290

Immunogen

Synthetic peptide within Human LRP1 aa 4,471-4,520

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809253

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

CD91 is a 600 kDa type I transmembrane protein composed of an alpha and beta chain. The alpha chain interacts with a variety of structurally and functionally diverse ligands, while the beta chain contains two tyrosine phosphorylation motifs that interact with adaptor and signaling proteins. This receptor is expressed by cells in the monocytic lineage, dendritic cells, hepatocytes, fibroblasts, adipocytes, neurons, and syncytiotrophoblasts. CD91 plays a crucial role in several cellular processes, including ligand endocytosis and trafficking to lysosomes, intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. It is involved in cross-presenting antigens via MHC class I molecules on antigen-presenting cells after endocytosis of heat shock protein-peptide complexes. Additionally, CD91 is essential for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of CD91 decreases with age and is found to be lower in brain tissue from Alzheimer patients compared to controls. The receptor also plays a role in cell migration and activating signaling cascades, highlighting its importance in both immune function and disease processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Signal transduction and targeted therapy

Shi X,Wang Z,Ren W,Chen L,Xu C,Li M,Fan S,Xu Y,Chen M,Zheng F,Zhang W,Zhou X,Zhang Y,Qiu S,Wu L,Zhou P,Lv X,Cui T,Qiao Y,Zhao H,Guo W,Chen W,Li S,Zhong W,Lin J,Yang S

Published figure using LRP1 recombinant monoclonal antibody (Product # MA5-31959) in Immunocytochemistry

Fri Jun 10 00:00:00 UTC 2022

Signal transduction and targeted therapy

Shi X,Wang Z,Ren W,Chen L,Xu C,Li M,Fan S,Xu Y,Chen M,Zheng F,Zhang W,Zhou X,Zhang Y,Qiu S,Wu L,Zhou P,Lv X,Cui T,Qiao Y,Zhao H,Guo W,Chen W,Li S,Zhong W,Lin J,Yang S

Published figure using LRP1 recombinant monoclonal antibody (Product # MA5-31959) in Immunocytochemistry

Fri Jun 10 00:00:00 UTC 2022

Signal transduction and targeted therapy

Shi X,Wang Z,Ren W,Chen L,Xu C,Li M,Fan S,Xu Y,Chen M,Zheng F,Zhang W,Zhou X,Zhang Y,Qiu S,Wu L,Zhou P,Lv X,Cui T,Qiao Y,Zhao H,Guo W,Chen W,Li S,Zhong W,Lin J,Yang S

Published figure using LRP1 recombinant monoclonal antibody (Product # MA5-31959) in Immunocytochemistry

Fri Jun 10 00:00:00 UTC 2022

CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins.

The Journal of clinical investigation

Shimizu-Albergine M,Basu D,Kanter JE,Kramer F,Kothari V,Barnhart S,Thornock C,Mullick AE,Clouet-Foraison N,Vaisar T,Heinecke JW,Hegele RA,Goldberg IJ,Bornfeldt KE

MA5-31959 was used in Western Blot to study how CREBH is influenced through APOE-dependent pathways in a mouse-model of type 1 diabetes mellitus.

Mon Nov 15 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: A2MR; alpha 2-macroglobulin receptor; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; lipoprotein receptor-related protein; low density lipoprotein receptor-related protein 1; low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor); LRP-1; lrp1; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor

Gene Aliases: A2MR; AI316852; APOER; APR; b2b1554Clo; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5

UniProt ID: (Human) Q07954, (Mouse) Q91ZX7, (Rat) G3V928

Entrez Gene ID: (Human) 4035, (Mouse) 16971, (Rat) 299858

Function(s)

protease binding receptor activity low-density lipoprotein receptor activity calcium ion binding protein binding protein complex binding apolipoprotein binding lipoprotein transporter activity poly(A) RNA binding lipoprotein particle receptor binding metal ion binding apolipoprotein extracellular matrix structural protein transmembrane signal receptor

Process(es)

retinoid metabolic process receptor-mediated endocytosis protein kinase C-activating G-protein coupled receptor signaling pathway aging cell proliferation positive regulation of cholesterol efflux negative regulation of neuron projection development negative regulation of smooth muscle cell migration cerebral cortex development negative regulation of Wnt signaling pathway positive regulation of lipid transport regulation of cholesterol transport regulation of phospholipase A2 activity regulation of actin cytoskeleton organization aorta morphogenesis lipoprotein metabolic process lipoprotein transport apoptotic cell clearance negative regulation of neuron apoptotic process positive regulation of protein transport beta-amyloid clearance negative regulation of platelet-derived growth factor receptor-beta signaling pathway endocytosis multicellular organism development cholesterol metabolic process

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LRP1 Recombinant Rabbit Monoclonal Antibody (SA0290)

LRP1 Antibody (MA5-31959) in ICC/IF

Product Details

MA5-31959

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions