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Application
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LYPD3 Antibody (MA5-29407) in ICC/IF
Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with LYPD3 Recombinant Rabbit Monoclonal Antibody (213) (Product # MA5-29407, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Product Details
MA5-29407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
213
Immunogen
Recombinant Human C4.4A/LYPD3 protein (Met1-His286)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785274
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human C4.4A/LYPD3.
Target Information
Lymphocyte antigen 6 family member D3 (LYPD3), also known as C4.4A, is a gene located on chromosome 19q13.11 that encodes a glycosylphosphatidylinositol (GPI)-anchored protein belonging to the Ly6/uPAR superfamily. LYPD3 is expressed in various epithelial tissues and is implicated in cellular adhesion and migration processes. The protein plays a significant role in tumor progression and metastasis, with its expression being notably upregulated in several cancers, including squamous cell carcinoma and lung cancer. LYPD3 contributes to the invasive behavior of cancer cells by interacting with matrix components and modulating signaling pathways related to cell motility and adhesion. Its involvement in cancer biology makes LYPD3 a potential biomarker for tumor aggressiveness and a target for therapeutic intervention aimed at inhibiting cancer cell dissemination. Research continues to explore its functional mechanisms in oncogenesis and potential applications in cancer treatment strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310061G07Rik; GPI-anchored metastasis-associated protein C4.4A homolog; GPI-anchored metastasis-associated protein homolog; Ly6/PLAUR domain-containing protein 3; Matrigel-induced gene C4 protein; MIG-C4
Gene Aliases: C4.4A; LYPD3; UNQ491/PRO1007
UniProt ID: (Human) O95274
Entrez Gene ID: (Human) 27076
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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