Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Primary Antibodies
  • Search All
  • Popular Product Areas
  • TaqMan Assays
  • Primary Antibodies
  • Secondary Antibodies
  • Isotype Controls
  • Proteins & Peptides
  • ELISA Kits
  • All Documents & Support
  • Certificates
  • SDS
  • Manuals & Protocols
  • Product FAQs
    Search button Close
            • Order Status
            • Quick Order
            • Sign in
              Sign in
              Don't have an account ? Create Account
              • Account
              • Check Order Status
              • Aspire Member Program
              • Connect: Lab, Data, Apps
              • Custom Products & Projects
              • Services Central

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Primary Antibodies ›
            • LDHA Antibodies

            Rockland

            Lactate Dehydrogenase Polyclonal Antibody, Biotin

            View all (64) LDHA antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite Lactate Dehydrogenase Polyclonal Antibody, Biotin

            • Antibody Testing Data (1)
            Application
            Lactate Dehydrogenase Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            Lactate Dehydrogenase Antibody (200-1673-0100) in ICC/IF

            Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT.... View More
            View Product

            Lactate Dehydrogenase Antibody in Immunocytochemistry (ICC/IF)

            Product Details

            200-1673-0100

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,500
            -

            Immunocytochemistry (ICC/IF)

            1:200
            -

            ELISA (ELISA)

            1:2,500-1:6,500
            -

            Immunoprecipitation (IP)

            1:500-1:2,500
            -
            Product Specifications

            Species Reactivity

            Rabbit

            Host/Isotype

            Goat / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
            View immunogen

            Conjugate

            Biotin

            Form

            Lyophilized

            Concentration

            1 mg/mL

            Purification

            Ion-exchange chromatography

            Storage buffer

            0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

            Contains

            0.01% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

            Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.

            Target Information

            Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

            Have you cited this product in a publication?
            Let us know so we can reference it here.
            Cite this product

            Bioinformatics

            Protein Aliases: L-lactate dehydrogenase; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase-M; lactate dehydrogenase-M (EC 1.1.1.27); Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH-A; LDH-M; OTTHUMP00000165206

            View more View less

            Gene Aliases: LDH-M; LDHA

            View more View less

            UniProt ID: (Rabbit) P13491

            View more View less

            Entrez Gene ID: (Rabbit) 100009107

            View more View less

            Function(s)

            dehydrogenase

            It has to be done as per old AB suggested Products section.
            guarantee_icon

            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
            help_icon

            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

            Contact tech support
            Ordering Plus Icon Minus Icon
            • Order Status
            • Order Help
            • Quick Order
            • Supply Center
            • eProcurement
            Support Plus Icon Minus Icon
            • Help and Support
            • Contact Us
            • Technical Support Centers
            • Documents and Certificates
            • Report a Site Issue
            Resources Plus Icon Minus Icon
            • Learning Centers
            • Promotions
            • Events and Webinars
            • Social Media
            About Thermo Fisher Plus Icon Minus Icon
            • About Us
            • Careers
            • Investors
            • News
            • Social Responsibility
            • Trademarks
            • Consumer Health Data Privacy Policy
            Our Portfolio Plus Icon Minus Icon
            • Thermo Scientific
            • Applied Biosystems
            • Invitrogen
            • Gibco
            • Ion Torrent
            • Fisher Scientific
            • Unity Lab Services
            • Patheon
            • PPD
            • Terms & Conditions
            • Privacy Information Center
            • Price & Freight Policy
            • Cookie Preferences

              Your choices regarding cookies on this site

              We and our affiliates and vendors use cookies and similar technologies to operate our sites, recognize visitors to our sites, provide secure log-in, collect statistics to optimize site functionality, and deliver content tailored to your interests. Click Accept All to accept all cookies and go directly to the site, click Reject All to reject all but cookies strictly necessary to the functioning of this site (required cookies), or click on Manage Settings to see detailed descriptions of the types of cookies and choose whether to accept certain cookies while on the site.
            © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
            United States flag icon
            United States

            Your items have has been added!


            Host server : magellan-search-green-56756dfb8b-7vhdw:80/100.66.79.199:80.
            git-commit: de6905f249d25e22729e613aef254bb544bd8b7c
            git-url: https://github.com/thermofisher/magellan-search
            git-branch: release/2.31.1-2025.06.25-1.0