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Product Details
MUB1101L1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
133A2
Immunogen
Lamin A
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mL/Test
Purification
Protein A/G
Storage buffer
PBS with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa lamin; lamin A; lamin A/C-like 1; lamin C; lamin C2; lamin-A/C; OTTHUMP00000015843; OTTHUMP00000015846; OTTHUMP00000015847; Prelamin-A/C; renal carcinoma antigen NY-REN-32; unnamed protein product
Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
UniProt ID: (Human) P02545, (Mouse) P48678
Entrez Gene ID: (Bovine) 404144, (Human) 4000, (Dog) 480124, (Mouse) 16905, (Rat) 60374
nucleus organization
nuclear envelope organization
muscle organ development
establishment of cell polarity
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
protein localization to nucleus
sterol regulatory element binding protein import into nucleus
ventricular cardiac muscle cell development
negative regulation of release of cytochrome c from mitochondria
positive regulation of cell aging
regulation of protein localization to nucleus
negative regulation of extrinsic apoptotic signaling pathway
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
spermatogenesis
response to mechanical stimulus
IRE1-mediated unfolded protein response
positive regulation of osteoblast differentiation
cellular response to hypoxia
negative regulation of adipose tissue development
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