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Invitrogen
Lamin A Monoclonal Antibody (4A4-A4), eBioscience™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Lamin A Antibody (14-9848-82) in ICC/IF
Immunofluorescent analysis of Lamin A (green) in A549 cells (80% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at 37730C and blocked with 10% Normal Donkey Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with 0.06 µg/mL Lamin A Mouse Monoclonal Antibody or 0.06 µg/mL Mouse IgG1 Isotype Control (Product # 14-4714-82) at 4730C, overnight, in blocking buffer, followed by Goat-anti Mouse IgG, Alexa Fluor P... View More
Product Details
14-9848-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4A4-A4
Immunogen
KLH-conjugated peptide corresponding to the C-terminus of human Lamin A
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815065
Product Specific Information
Description: This 4A4-A4 monoclonal antibody recognizes human and mouse mature Lamin A.
Applications Reported: This 4A4-A4 antibody has been reported for use in western blotting, immunocytochemistry and immunoprecipitation.
Applications Tested: This 4A4-A4 antibody has been tested by western blotting of human cell line lysates. This may be used at 0.2 µg/mL. This 4A4-A4 antibody has also been tested by immunocytochemistry on A549 cells. This may be used at 0.06 µg/mL.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa lamin; lamin A/C-like 1; lamin C; lamin-A/C; OTTHUMP00000015843; OTTHUMP00000015846; OTTHUMP00000015847; Prelamin-A/C; renal carcinoma antigen NY-REN-32
Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
UniProt ID: (Human) P02545, (Mouse) P48678
Entrez Gene ID: (Human) 4000, (Mouse) 16905
nucleus organization
nuclear envelope organization
muscle organ development
establishment of cell polarity
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
protein localization to nucleus
sterol regulatory element binding protein import into nucleus
ventricular cardiac muscle cell development
negative regulation of release of cytochrome c from mitochondria
positive regulation of cell aging
regulation of protein localization to nucleus
negative regulation of extrinsic apoptotic signaling pathway
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
spermatogenesis
response to mechanical stimulus
IRE1-mediated unfolded protein response
positive regulation of osteoblast differentiation
cellular response to hypoxia
negative regulation of adipose tissue development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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