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Laminin Antibody (PA5-115490) in ICC/IF
Immunocytochemistry analysis of Laminin in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115490) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LAMA2(Accession P24043), corresponding to amino acid residues Q383-A433.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900126
Product Specific Information
Antibody detects endogenous levels of total Laminin 2 alpha.
Target Information
Laminin antibodies are widely used to label blood vessels and basement membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dystrophia muscularis; Laminin A chain; Laminin B1 chain; laminin B1 subunit 1; Laminin B2 chain; Laminin M chain; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit gamma-1; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, gamma 1 (formerly LAMB2); laminin-1; Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit alpha; Laminin-12 subunit beta; Laminin-2 subunit alpha; Laminin-2 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit alpha; Laminin-3 subunit gamma; Laminin-4 subunit alpha; Laminin-4 subunit gamma; Laminin-6 subunit beta; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit beta; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Merosin heavy chain; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma
Gene Aliases: 5830440B04; AA408497; B2e; C77966; C80098; C81607; CLM; D130003D08Rik; dy; LAMA; Lama-1; LAMA1; LAMA2; Lamb-1; LAMB1; Lamb1-1; LAMB2; LAMC1; LAMM; LIS5; mer; merosin; mKIAA4087; PTBHS; S-LAM-alpha
UniProt ID: (Human) P25391, (Human) P24043, (Human) P07942, (Human) P11047, (Mouse) P19137, (Mouse) Q60675, (Mouse) P02469
Entrez Gene ID: (Human) 284217, (Human) 3908, (Human) 3912, (Human) 3915, (Mouse) 16772, (Rat) 316758, (Rat) 309368, (Mouse) 16773, (Mouse) 16777, (Rat) 298941, (Mouse) 226519, (Rat) 117036
cell adhesion
cell surface receptor signaling pathway
regulation of cell adhesion
extracellular matrix organization
regulation of cell migration
regulation of embryonic development
axon guidance
muscle organ development
myelination in peripheral nervous system
positive regulation of synaptic transmission, cholinergic
neuronal-glial interaction involved in cerebral cortex radial glia guided migration
positive regulation of cell migration
neuron projection development
substrate adhesion-dependent cell spreading
endodermal cell differentiation
odontogenesis
positive regulation of epithelial cell proliferation
protein complex assembly
endoderm development
cell migration
extracellular matrix disassembly
hemidesmosome assembly
morphogenesis of an epithelial sheet
tissue development
establishment of epithelial cell apical/basal polarity
retina development in camera-type eye
epithelial tube branching involved in lung morphogenesis
branching involved in salivary gland morphogenesis
retinal blood vessel morphogenesis
biological_process
negative regulation of cell adhesion
embryo implantation
learning or memory
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