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            • Primary Antibodies ›
            • Laminin gamma-1,2,3 Antibodies

            Invitrogen

            Laminin gamma-1,2,3 Polyclonal Antibody

            Advanced Verification
            View all (2) Laminin gamma-1,2,3 antibodies
            Datasheet
            Protocols
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            Datasheet
            Protocols
            Questions & Answers

            Cite Laminin gamma-1,2,3 Polyclonal Antibody

            • Antibody Testing Data (3)
            • Advanced Verification (2)
            Application
            Laminin gamma-1,2,3 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 5

            Laminin gamma-1,2,3 Antibody (PA5-79579) in ICC/IF

            Immunofluorescence analysis of Laminin gamma-1,2,3 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Laminin gamma-1,2,3 Polyclonal Antibody (Product # PA5-79580) at 1:100 dilution in 0.1% BSA, incubated... View More
            View Product

            Laminin gamma-1,2,3 Antibody in Immunocytochemistry (ICC/IF)
            Laminin gamma-1,2,3 Antibody in Western Blot (WB)
            Laminin gamma-1,2,3 Antibody in Western Blot (WB)
            Laminin gamma-1,2,3 Antibody
            Laminin gamma-1,2,3 Antibody

            Product Details

            PA5-79579

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.1-0.5 µg/mL
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            0.5-1 µg/mL
            -

            Immunohistochemistry (Frozen) (IHC (F))

            0.5-1 µg/mL
            -

            Immunocytochemistry (ICC/IF)

            0.5-1 µg/mL
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ).
            View immunogen

            Conjugate

            Unconjugated

            Form

            Lyophilized

            Concentration

            500 µg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with 5mg BSA

            Contains

            0.05mg sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2746694

            Product Specific Information

            This antibody reacts will all 3 isoforms of Laminin gamma: Laminin gamma-1, gamma-2 and gamma-3.

            Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

            Positive Control - WB: human A431 whole cell, human A549 whole cell, human PC-3 whole cell, human Caco-2 whole cellrat NRK whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse Hepa1-6 whole cell. IHC: human lung cancer tissue IHC-F: human placenta tissue.

            Target Information

            Extracellular matrix (ECM) surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains Dystroglycan is a major laminin receptor in muscle and epithelia, and nidogen is a high-affinity laminin-binding protein important for basement-membrane assembly.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: alternatively spliced laminin gamma2 chain; BM600-100kDa; Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; lamimin, gamma 2; Laminin B2 chain; Laminin B2t chain; laminin gamma2 chain; Laminin subunit gamma-1; Laminin subunit gamma-2; Laminin subunit gamma-3; laminin, gamma 1 (formerly LAMB2); laminin, gamma 2; laminin, gamma 3; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit gamma; Laminin-14 subunit gamma; Laminin-15 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-5 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma; nicein, 100kD; nicein, 100kDa; S-LAM gamma; S-laminin subunit gamma

            View more View less

            Gene Aliases: AA589349; AI562206; AW240805; B2e; B2T; BM600; CSF; EBR2; EBR2A; LAMB2; LAMB2T; LAMC1; LAMC2; LAMC3; LAMNB2; OCCM

            View more View less

            UniProt ID: (Human) P11047, (Human) Q13753, (Human) Q9Y6N6, (Mouse) Q4VAI3

            View more View less

            Entrez Gene ID: (Human) 3915, (Human) 3918, (Human) 10319, (Mouse) 226519, (Rat) 117036, (Mouse) 16782, (Rat) 192362, (Rat) 311862, (Mouse) 23928

            View more View less

            Function(s)

            extracellular matrix structural constituent heparin binding structural molecule activity protein binding glycosphingolipid binding molecular_function extracellular matrix protein DNA metabolism protein

            Process(es)

            protein complex assembly cell adhesion endoderm development cell migration extracellular matrix disassembly extracellular matrix organization hemidesmosome assembly substrate adhesion-dependent cell spreading positive regulation of epithelial cell proliferation epidermis development cell morphogenesis involved in differentiation visual perception astrocyte development retina development in camera-type eye neuron projection development biological_process
            It has to be done as per old AB suggested Products section.
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