Hamburger Menu Button
Thermo Fisher Scientific Logo
登入
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Tubes
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Clinical Genomics
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO 和 CRO 服務
    • CDMO 服務
    • 臨床試驗 CRO 服務
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • Register for an Account
    • How to Order
    • Instrument Support
    • Technical Support Centers
    • Learning Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Primary Antibodies
  • Search All
  • Popular Product Areas
  • TaqMan Assays
  • Primary Antibodies
  • Secondary Antibodies
  • Isotype Controls
  • Proteins and Peptides
  • ELISA Kits
  • All Documents & Support
  • Certificates
  • SDS
  • Product FAQs
  • Documents & Support
  • Manuals & Protocols
    Search button Close
            Target of Interest
            Application
            Target Species
            • Order Status
            • 快速訂購
            • 登入
              登入
              Don't have an account ? Create Account
              • 帳戶
              • 訂單
              • 訂製產品與專案

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Primary Antibodies ›
            • Ly-6A/E (Sca-1) Antibodies

            Invitrogen

            Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™

            94 Published Figures
            96 References
            View all (34) Ly-6A/E (Sca-1) antibodies

            Promotions

            View available promotion(s)

            Promo Code: P5877545 Don't miss out: buy 3, only pay for 2 Extended to June 20: take advantage of our 3 for 2 promotion. Whether you are restocking essentials or trying something new, now is a great time to get more for your money!. Learn more

            Promo Code: RPUZZ25 Stock up on essentials to piece your discovery together Until June 27, save up to $650 and get an exclusive lab-themed hidden-object puzzle. Learn more

            Datasheet
            Protocols
            Questions & Answers
            Tech Support
            Catalog # 62-5981-82
            Web quote
            You can generate a web quote (PDF) by signing in to your account and adding products to your cart. View cart and click the 'Generate a web quote' button. With your web quote document, you can get approval for your order and complete your order online.
             
            Price (HKD)
            4,333.00
            100 µg
             
            Check your price & availability
            Request bulk or custom format
            Datasheet
            Protocols
            Questions & Answers
            Tech Support

            Cite Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (94)
            Application
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 95

            Ly-6A/E (Sca-1) Antibody (62-5981-82) in Flow

            Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1172-82) and 0.125 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436 (right). Cells in the large scatter population were used for analysis.
            View Product

            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry (ICC/IF)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry (ICC/IF)
            Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
            Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
            Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
            Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
            Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
            Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)

            Product Details

            62-5981-82

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 6 publications 6 publications

            Immunocytochemistry (ICC/IF)

            -
            View 5 publications 5 publications

            Flow Cytometry (Flow)

            0.25 µg/test
            View 85 publications 85 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Fish, Human, Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            D7

            Conjugate

            Super Bright™ 436
            • Unconjugated
            • Alexa Fluor 700 
            • APC 
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 711
            • Brilliant Violet 786
            • FITC
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • Super Bright 600
            • Super Bright 645
            • Request custom conjugation

            Excitation/Emission Max

            413/431 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2, with BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2637287

            Product Specific Information

            Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

            Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

            When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

            Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

            Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

            Target Information

            Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
            Video Player is loading.
            Current Time 0:00
            /
            Duration 0:00
            Loaded: 0%
            0:00
            Stream Type LIVE
            Remaining Time -0:00
             
            1x
            • 2x
            • 1.75x
            • 1.5x
            • 1.25x
            • 1x, selected
            • 0.75x
            • 0.5x
            • Chapters
            • descriptions off, selected
            • captions settings, opens captions settings dialog
            • captions off, selected

              This is a modal window.

              Beginning of dialog window. Escape will cancel and close the window.

              Text
              Text Background
              Caption Area Background
              Font Size
              Text Edge Style
              Font Family

              End of dialog window.

              This is a modal window. This modal can be closed by pressing the Escape key or activating the close button.

              How to use the Panel Builder

              Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

              Bioinformatics

              Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

              View more View less

              Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

              View more View less

              UniProt ID: (Mouse) P05533, (Mouse) Q64253

              View more View less

              Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

              View more View less

              Process(es)

              in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis
              It has to be done as per old AB suggested Products section.
              guarantee_icon

              Performance Guarantee

              If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

              Learn more
              help_icon

              We're here to help

              Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

              Contact tech support
              Ordering Plus Icon Minus Icon
              • Order Status
              • Order Help
              • Quick Order
              • Supply Center
              • eProcurement
              Support Plus Icon Minus Icon
              • Help and Support
              • Contact Us
              • Technical Support Centers
              • Documents and Certificates
              • Report a Site Issue
              Resources Plus Icon Minus Icon
              • Learning Centers
              • Promotions
              • Events and Webinars
              • Social Media
              About Thermo Fisher Plus Icon Minus Icon
              • About Us About Us
              • Careers Careers
              • Investors Investors
              • News News
              • Social Responsibility Social Responsibility
              • Trademarks
              Our Portfolio Plus Icon Minus Icon
              • Thermo Scientific
              • Applied Biosystems
              • Invitrogen
              • Gibco
              • Ion Torrent
              • Fisher Scientific
              • Unity Lab Services
              • Patheon
              • PPD
              • Terms & Conditions
              • Privacy Policy
              • Price & Freight Policy
              • Cookie偏好

                在此網站使用 Cookie 的選擇

                我們以及我們的附屬公司和供應商使用 cookies 和類似技術來經營我們的網站、識別我們網站的訪客、提供安全登入、收集統計資料以優化網站功能,並提供根據您的興趣量身定制的內容。點擊「全部接受」 以接受所有 cookies,並直接進入網站,或點擊「管理設定」 以查看 cookies 類型的詳細說明,並選擇是否在網站接受某些 cookies。
              © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
              hong kong flag icon
              Hong Kong

              Your items have has been added!


              Host server : magellan-search-green-56756dfb8b-mgmkt:80/100.66.75.22:80.
              git-commit: de6905f249d25e22729e613aef254bb544bd8b7c
              git-url: https://github.com/thermofisher/magellan-search
              git-branch: release/2.31.1-2025.06.25-1.0