Invitrogen

Ly-6G Monoclonal Antibody (1A8-Ly6g), PerCP-eFluor™ 710, eBioscience™

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Antibody Testing Data (1)
Published Figures (18)

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Ly-6G Antibody (46-9668-82) in Flow

Staining of mouse bone marrow cells with Anti-Mouse Ly-6C Alexa Fluor® 488 (Product # 53-5932-82) and 0.06 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.06 µg of Anti-Mouse Ly-6G (Gr-1) PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

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Product Details

46-9668-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A8-Ly6g

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573893

Product Specific Information

Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.

Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.

Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Intranasal Delivery of Endothelial Cell-Derived Extracellular Vesicles with Supramolecular Gel Attenuates Myocardial Ischemia-Reperfusion Injury.

International journal of nanomedicine

Wang J,Tan Y,Dai Y,Hu K,Tan X,Jiang S,Li G,Zhang X,Kang L,Wang X,Xu B

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injury.

iScience

Gonzatti MB,Freire BM,Antunes MM,de Menezes GB,Talbot J,Peron JPS,Basso AS,Keller AC

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Neutrophil Extracellular Traps Release following Hypoxic-Ischemic Brain Injury in Newborn Rats Treated with Therapeutic Hypothermia.

International journal of molecular sciences

Bernis ME,Zweyer M,Maes E,Schleehuber Y,Sabir H

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Fri Feb 10 00:00:00 EST 2023

LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer.

Journal for immunotherapy of cancer

Zhao T,Liu S,Hanna NH,Jalal S,Ding X,Wan J,Yan C,Du H

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE.

Nature

Pernet E,Sun S,Sarden N,Gona S,Nguyen A,Khan N,Mawhinney M,Tran KA,Chronopoulos J,Amberkar D,Sadeghi M,Grant A,Wali S,Prevel R,Ding J,Martin JG,Thanabalasuriar A,Yipp BG,Barreiro LB,Divangahi M

46-9668-82 was used in Flow cytometry/Cell sorting to highlight the complexity of prenatal RTM programming and reveal their dependency on in trans eicosanoid production by neutrophils for lifelong self-renewal.

Wed Feb 01 00:00:00 EST 2023

GPR84 regulates pulmonary inflammation by modulating neutrophil functions.

Acta pharmacologica Sinica

Wang SW,Zhang Q,Lu D,Fang YC,Yan XC,Chen J,Xia ZK,Yuan QT,Chen LH,Zhang YM,Nan FJ,Xie X

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

46-9668-82 was used in Flow cytometry/Cell sorting to show that stimulation of S.tm-infected macrophages with IL-4 reduced bacterial multiplication via metabolic re-programming of L-arginine-dependent pathways.

Fri Apr 14 00:00:00 EDT 2023

A Mouse Infection Model with a Wildtype Salmonella enterica Serovar Typhimurium Strain for the Analysis of Inflammatory Innate Immune Cells.

Bio-protocol

Pfeifhofer-Obermair C,Brigo N,Tymoszuk P,Weiss AG

46-9668-82 was used in Flow cytometry/Cell sorting to establish a robust experimental model for the characterization of these cell types and their response during host-pathogen interactions.

Tue Apr 05 00:00:00 EDT 2022

Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein.

Bio-protocol

Brigo N,Pfeifhofer-Obermair C,Demetz E,Tymoszuk P,Weiss G

46-9668-82 was used in Flow cytometry/Cell sorting to suggest a method that identifies pro- and anti-inflammatory bone marrow-derived macrophage subtypes, during an infection with Salmonella enterica serovar Typhimurium.

Sun Jun 05 00:00:00 EDT 2022

Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.

Cell host & microbe

Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Wed Feb 10 00:00:00 EST 2021

Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice.

Muscle & nerve

Kawanishi N,Machida S

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

G-MDSCs promote aging-related cardiac fibrosis by activating myofibroblasts and preventing senescence.

Cell death & disease

Sun SN,Ni SH,Li Y,Liu X,Deng JP,Chen ZX,Li H,Feng WJ,Huang YS,Li DN,Xian SX,Yang ZQ,Wang LJ,Lu L

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

CCR2 deficiency alters activation of microglia subsets in traumatic brain injury.

Cell reports

Somebang K,Rudolph J,Imhof I,Li L,Niemi EC,Shigenaga J,Tran H,Gill TM,Lo I,Zabel BA,Schmajuk G,Wipke BT,Gyoneva S,Jandreski L,Craft M,Benedetto G,Plowey ED,Charo I,Campbell J,Ye CJ,Panter SS,Nakamura MC,Eckalbar W,Hsieh CL

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Interleukin-4 Programmed Macrophages Suppress Colitis and Do Not Enhance Infectious-Colitis, Inflammation-Associated Colon Cancer or Airway Hypersensitivity.

Frontiers in immunology

Callejas BE,Blyth GAD,Jendzjowsky N,Wang A,Babbar A,Koro K,Wilson RJA,Kelly MM,Cobo ER,McKay DM

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Local sympathetic innervations modulate the lung innate immune responses.

Science advances

Liu T,Yang L,Han X,Ding X,Li J,Yang J

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Bivalent Inhibitor with Selectivity for Trimeric MMP-9 Amplifies Neutrophil Chemotaxis and Enables Functional Studies on MMP-9 Proteoforms.

Cells

Nuti E,Rossello A,Cuffaro D,Camodeca C,Van Bael J,van der Maat D,Martens E,Fiten P,Pereira RVS,Ugarte-Berzal E,Gouwy M,Opdenakker G,Vandooren J

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Tue Jul 07 00:00:00 EDT 2020

IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A⁺Foxp3⁺ Cells During Mucosal Infection and Is Dysregulated With Aging.

Frontiers in immunology

Bhaskaran N,Faddoul F,Paes da Silva A,Jayaraman S,Schneider E,Mamileti P,Weinberg A,Pandiyan P

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A⁺Foxp3⁺ Cells During Mucosal Infection and Is Dysregulated With Aging.

Frontiers in immunology

Bhaskaran N,Faddoul F,Paes da Silva A,Jayaraman S,Schneider E,Mamileti P,Weinberg A,Pandiyan P

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Loss of Atg7 causes chaotic nucleosome assembly of mouse bone marrow CD11b⁺Ly6G^- myeloid cells.

Aging

Fang Y,Gu Y,Li L,Zhu L,Qian J,Zhao C,Xu L,Wei W,Du Y,Yuan N,Zhang S,Yuan Y,Xu Y,Jiang C,Wang J

46-9668 was used in Flow cytometry/Cell sorting to suggest that Atg7 is essential for maintaining proper nucleosome assembly and limiting aging in the bone marrow CD11b+Ly6G- population.

Tue Nov 24 00:00:00 EST 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Transformation of Fonsecaea pedrosoi into sclerotic cells links to the refractoriness of experimental chromoblastomycosis in BALB/c mice via a mechanism involving a chitin-induced impairment of IFN-γ production.

PLoS neglected tropical diseases

Dong B,Tong Z,Li R,Chen SC,Liu W,Liu W,Chen Y,Zhang X,Duan Y,Li D,Chen L

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration.

Cell research

Cai Z,Zhang A,Choksi S,Li W,Li T,Zhang XM,Liu ZG

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

ATP-Induced Inflammasome Activation and Pyroptosis Is Regulated by AMP-Activated Protein Kinase in Macrophages.

Frontiers in immunology

Zha QB,Wei HX,Li CG,Liang YD,Xu LH,Bai WJ,Pan H,He XH,Ouyang DY

Published figure using Ly-6G monoclonal antibody (Product # 46-9668-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Cystic fibrosis-adapted Pseudomonas aeruginosa quorum sensing lasR mutants cause hyperinflammatory responses.

Science advances

LaFayette SL,Houle D,Beaudoin T,Wojewodka G,Radzioch D,Hoffman LR,Burns JL,Dandekar AA,Smalley NE,Chandler JR,Zlosnik JE,Speert DP,Bernier J,Matouk E,Brochiero E,Rousseau S,Nguyen D

46-9668 was used in Flow cytometry/Cell sorting to investigate how mutations in lasR alter host-pathogen relationships, showing that Cystic fibrosis-adapted Pseudomonas aeruginosa lasR mutants cause hyperinflammatory responses.

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Ly-6G; Ly-6G.1; Lymphocyte antigen 6G

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Gene Aliases: Gr-1; Gr1; Ly-6G; Ly6g

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UniProt ID: (Mouse) P35461

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Entrez Gene ID: (Mouse) 546644

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ly-6G Monoclonal Antibody (1A8-Ly6g), PerCP-eFluor™ 710, eBioscience™

Ly-6G Antibody (46-9668-82) in Flow

Product Details

46-9668-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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