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Invitrogen
M13 phage coat protein Monoclonal Antibody (A5B3), HRP
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Application
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M13 phage coat protein Antibody (MA5-36125) in ELISA
ELISA of M13 using an M13 monoclonal antibody (Product # MA5-36125). Samples were blocked with 1% BSA for 1 hour at 37°C and incubated with the primary antibody at a dilution of 1:10,000 for 1 hour at 25°C. The M13 antibody was repeatedly frozen and thawed for 4 times at -80°C, 4°C, 37°C.
Product Details
MA5-36125
Product Specifications
Species Reactivity
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A5B3
Immunogen
Synthetic peptide within bacteriophage M13 aa 1-50
Conjugate
HRP
Form
Liquid
Concentration
2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890559
Target Information
M13 antibodies have numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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