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Invitrogen
Phospho-Chk1 (Ser345) Recombinant Rabbit Monoclonal Antibody (Chk1S345-R3F9), FITC
FIGURE: 1 / 1
Phospho-Chk1 (Ser345) Antibody (MA5-37021) in Flow
Flow cytometry of Phospho-Chk1 (Ser345) using HLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37021). Images correspond with the following conditions: untreated, unstained negative control (blue), untreated and stained (red), treated with UV/TPA and stained (green).
Product Details
MA5-37021
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Chk1S345-R3F9
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser345 of human phospho Chk1.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS with 0.20% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896956
Target Information
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1
Gene Aliases: C85740; CHEK1; CHK1; rad27
UniProt ID: (Human) O14757, (Mouse) O35280
Entrez Gene ID: (Human) 1111, (Mouse) 12649
DNA damage checkpoint
G2/M transition of mitotic cell cycle
DNA replication
DNA repair
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
regulation of double-strand break repair via homologous recombination
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
peptidyl-threonine phosphorylation
G2 DNA damage checkpoint
histone H3-T11 phosphorylation
regulation of cell proliferation
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
chromatin-mediated maintenance of transcription
cellular response to mechanical stimulus
replicative senescence
regulation of signal transduction by p53 class mediator
regulation of histone H3-K9 acetylation
protein phosphorylation
cell cycle
regulation of gene expression
negative regulation of G2/M transition of mitotic cell cycle
phosphorylation
negative regulation of DNA biosynthetic process
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