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Invitrogen
Phospho-PDPK1 (Ser241) Recombinant Rabbit Monoclonal Antibody (PDK1S241-F7)
FIGURE: 1 / 2
Phospho-PDPK1 (Ser241) Antibody (MA5-37088) in Flow
Flow cytometry of Phospho-PDPK1 (Ser241) using 293T cells. Samples were incubated in monoclonal Phospho-PDPK1 (Ser241) antibody (Product # MA5-37088) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (light blue), treated with K252 (red), with pervanadate (green), K252 and blocked with phospho-peptide (black), pervanadate and blocked with phospho peptide (gold),... View More
Product Details
MA5-37088
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PDK1S241-F7
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser241 of human phospho PDPK1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897022
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase like gene 1; PkB-like 1
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) Q6FI20
Entrez Gene ID: (Human) 5170
stimulatory C-type lectin receptor signaling pathway
type B pancreatic cell development
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
epidermal growth factor receptor signaling pathway
positive regulation of phospholipase activity
regulation of endothelial cell migration
negative regulation of cardiac muscle cell apoptotic process
cell migration
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
calcium-mediated signaling
actin cytoskeleton organization
platelet activation
negative regulation of transforming growth factor beta receptor signaling pathway
T cell costimulation
activation of protein kinase B activity
cellular response to insulin stimulus
negative regulation of toll-like receptor signaling pathway
intracellular signal transduction
Fc-epsilon receptor signaling pathway
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mast cell degranulation
protein autophosphorylation
phosphatidylinositol-mediated signaling
focal adhesion assembly
T cell receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
positive regulation of establishment of protein localization to plasma membrane
extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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