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Invitrogen
MANEA Polyclonal Antibody
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Application
FIGURE: 1 / 1
MANEA Antibody (PA5-88029) in WB
Western blot analysis of MANEA in extracts of various cell lines. Samples were incubated with MANEA Polyclonal antibody (Product # PA5-88029) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88029
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_0789172)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804600
Product Specific Information
Immunogen sequence: RPNTATFGAP FGLDLLPELH QRTIHLGKNF DFQKSDRINS ETNTKNLKSV EITMKPSKAS ELNLDELPPL N; Positive Samples: 293T, U-87MG; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 1,2-endomannosidase; Endo-alpha mannosidase; endomannosidase; Glycoprotein endo-alpha-1,2-mannosidase; Mandaselin; mannosidase, endo-alpha
Gene Aliases: ENDO; hEndo; MANEA
UniProt ID: (Human) Q5SRI9
Entrez Gene ID: (Human) 79694
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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